Laboratory for Bioinformatics, Graduate School of Systems Life Sciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Metab Eng. 2013 Nov;20:101-8. doi: 10.1016/j.ymben.2013.09.007. Epub 2013 Sep 25.
Production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. Here, we constructed a synthetic pathway composed of acetyl-CoA acetyl transferase (encoded by thl), acetoacetyl-CoA transferase (encoded by atoAD), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in Synechococcus elongatus strain PCC 7942 to produce isopropanol. The enzyme-coding genes, heterogeneously originating from Clostridium acetobutylicum ATCC 824 (thl and adc), Escherichia coli K-12 MG1655 (atoAD) and Clostridium beijerinckii (adh), were integrated into the S. elongatus genome. Under the optimized production conditions, the engineered cyanobacteria produced 26.5 mg/L of isopropanol after 9 days.
利用工程化蓝藻直接从太阳能和二氧化碳生产替代燃料或化学品是减少对石油依赖和最小化碳排放的一种有吸引力的方法。在这里，我们构建了一个由乙酰辅酶 A 乙酰基转移酶（由 thl 编码）、乙酰乙酰辅酶 A 转移酶（由 atoAD 编码）、乙酰乙酸脱羧酶（由 adc 编码）和二级醇脱氢酶（由 adh 编码）组成的合成途径，以在 Synechococcus elongatus 菌株 PCC 7942 中生产异丙醇。这些酶编码基因，异源来源于 Clostridium acetobutylicum ATCC 824（thl 和 adc）、Escherichia coli K-12 MG1655（atoAD）和 Clostridium beijerinckii（adh），被整合到 S. elongatus 基因组中。在优化的生产条件下，经过 9 天的时间，工程化蓝藻产生了 26.5mg/L 的异丙醇。
