Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute of Genomics and Proteomics, University of California Los Angeles, Boyer Hall, 611 Charles E. Young Dr. E, Los Angeles, CA, 90095-1570, USA.
Sci Rep. 2022 May 11;12(1):7700. doi: 10.1038/s41598-022-11653-3.
Ethanol is a widely available carbon compound that can be increasingly produced with a net negative carbon balance. Carbon-negative ethanol might therefore provide a feedstock for building a wider range of sustainable chemicals. Here we show how ethanol can be converted with a cell free system into acetyl-CoA, a central precursor for myriad biochemicals, and how we can use the energy stored in ethanol to generate ATP, another key molecule important for powering biochemical pathways. The ATP generator produces acetone as a value-added side product. Our ATP generator reached titers of 27 ± 6 mM ATP and 59 ± 15 mM acetone with maximum ATP synthesis rate of 2.8 ± 0.6 mM/h and acetone of 7.8 ± 0.8 mM/h. We illustrated how the ATP generating module can power cell-free biochemical pathways by converting mevalonate into isoprenol at a titer of 12.5 ± 0.8 mM and a maximum productivity of 1.0 ± 0.05 mM/h. These proof-of-principle demonstrations may ultimately find their way to the manufacture of diverse chemicals from ethanol and other simple carbon compounds.
乙醇是一种广泛存在的碳化合物，可以通过净碳平衡的方式越来越多地生产。因此，负碳乙醇可能为生产更广泛的可持续化学品提供原料。在这里，我们展示了如何使用无细胞系统将乙醇转化为乙酰辅酶 A，乙酰辅酶 A 是众多生化物质的中心前体，以及如何利用乙醇中储存的能量产生 ATP，ATP 是另一种对生化途径供能至关重要的关键分子。ATP 生成器会产生丙酮作为附加值的副产物。我们的 ATP 生成器的 ATP 产量达到 27±6 mM，丙酮产量达到 59±15 mM，最大 ATP 合成速率为 2.8±0.6 mM/h，丙酮产量为 7.8±0.8 mM/h。我们说明了如何通过将甲羟戊酸转化为异戊烯醇，在 12.5±0.8 mM 的产量和 1.0±0.05 mM/h 的最大生产率下，使用 ATP 生成模块为无细胞生化途径提供动力。这些原理验证演示最终可能会找到从乙醇和其他简单碳化合物制造各种化学品的方法。
