厌氧苯降解菌株DN11的降解能力及生物强化潜力
Degradative capacities and bioaugmentation potential of an anaerobic benzene-degrading bacterium strain DN11.
作者信息
Kasai Yuki, Kodama Yumiko, Takahata Yoh, Hoaki Toshihiro, Watanabe Kazuya
机构信息
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, 026-0001, Japan, and Taisei Corporation, 344-1 Nase, Totsuka-ku, Yokohama, 245-0051, Japan.
出版信息
Environ Sci Technol. 2007 Sep 1;41(17):6222-7. doi: 10.1021/es062842p.
Azoarcus sp. strain DN11 is a denitrifying bacterium capable of benzene degradation under anaerobic conditions. The present study evaluated strain DN11 for its application to bioaugmentation of benzene-contaminated underground aquifers. Strain DN11 could grow on benzene, toluene, m-xylene, and benzoate as the sole carbon and energy sources under nitrate-reducing conditions, although o- and p-xylenes were transformed in the presence of toluene. Phenol was not utilized under anaerobic conditions. Kinetic analysis of anaerobic benzene degradation estimated its apparent affinity and inhibition constants to be 0.82 and 11 microM, respectively. Benzene-contaminated groundwater taken from a former coal-distillation plant site was anaerobically incubated in laboratory bottles and supplemented with either inorganic nutrients (nitrogen, phosphorus, and nitrate) alone, or the nutrients plus strain DN11, showing that benzene was significantly degraded only when DN11 was introduced. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments, and quantitative PCR revealed that DN11 decreased after benzene was degraded. Following the decrease in DN11 16S rRNA gene fragments corresponding to bacteria related to Owenweeksia hongkongensis and Pelotomaculum isophthalicum, appeared as strong bands, suggesting possible metabolic interactions in anaerobic benzene degradation. Results suggest that DN11 is potentially useful for degrading benzene that contaminates underground aquifers at relatively low concentrations.
偶氮弧菌属菌株DN11是一种能在厌氧条件下进行苯降解的反硝化细菌。本研究评估了菌株DN11在苯污染地下含水层生物强化中的应用。菌株DN11在硝酸盐还原条件下能够以苯、甲苯、间二甲苯和苯甲酸盐作为唯一碳源和能源生长，不过邻二甲苯和对二甲苯在甲苯存在时会被转化。苯酚在厌氧条件下不能被利用。厌氧苯降解的动力学分析估计其表观亲和力和抑制常数分别为0.82和11微摩尔。从一个 former coal-distillation plant site 采集的受苯污染的地下水在实验室瓶中进行厌氧培养，并单独添加无机营养物（氮、磷和硝酸盐），或添加营养物和菌株DN11，结果表明只有引入DN11时苯才会被显著降解。对PCR扩增的16S rRNA基因片段进行变性梯度凝胶电泳和定量PCR分析表明，苯降解后DN11数量减少。在与香港欧文氏菌和间苯二甲酸泥杆菌相关的细菌对应的DN11 16S rRNA基因片段数量减少后，出现了强条带，这表明在厌氧苯降解过程中可能存在代谢相互作用。结果表明，DN11在降解相对低浓度污染地下含水层的苯方面具有潜在用途。 （注：原文中“former coal-distillation plant site”表述不太准确，可能是“former coal-distillation plant”，意思是“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant”，即“ former coal-distillation plant site”，可能是“ former coal-distillation plant”，意思是“ former coal-distillation plant”，即“ former 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