重复的WG/GW模体在RNAi相关组件中形成功能和进化上保守的AGO结合平台。
Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components.
作者信息
El-Shami Mahmoud, Pontier Dominique, Lahmy Sylvie, Braun Laurence, Picart Claire, Vega Danielle, Hakimi Mohamed-Ali, Jacobsen Steven E, Cooke Richard, Lagrange Thierry
机构信息
Laboratoire Génome et Développement de Plantes, Centre National de la Recherche Scientifique/Institut de Recherche pour le Développement/Université de Perpignan 5096, 66860 Perpignan Cedex, France.
出版信息
Genes Dev. 2007 Oct 15;21(20):2539-44. doi: 10.1101/gad.451207.
Two forms of RNA Polymerase IV (PolIVa/PolIVb) have been implicated in RNA-directed DNA methylation (RdDM) in Arabidopsis. Prevailing models imply a distinct function for PolIVb by association of Argonaute4 (AGO4) with the C-terminal domain (CTD) of its largest subunit NRPD1b. Here we show that the extended CTD of NRPD1b-type proteins exhibits conserved Argonaute-binding capacity through a WG/GW-rich region that functionally distinguishes Pol IVb from Pol IVa, and that is essential for RdDM. Site-specific mutagenesis and domain-swapping experiments between AtNRPD1b and the human protein GW182 demonstrated that reiterated WG/GW motifs form evolutionarily and functionally conserved Argonaute-binding platforms in RNA interference (RNAi)-related components.
两种形式的RNA聚合酶IV（PolIVa/PolIVb）参与了拟南芥中的RNA指导的DNA甲基化（RdDM）过程。流行的模型认为，通过AGO4与最大亚基NRPD1b的C末端结构域（CTD）结合，PolIVb具有独特的功能。在这里，我们表明，NRPD1b型蛋白的扩展CTD通过富含WG/GW的区域表现出保守的AGO结合能力，该区域在功能上区分了Pol IVb和Pol IVa，并且对RdDM至关重要。AtNRPD1b与人类蛋白GW182之间的位点特异性诱变和结构域交换实验表明，重复的WG/GW基序在RNA干扰（RNAi）相关组分中形成了进化上和功能上保守的AGO结合平台。
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