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[2006年北京居民中流行的HIV-1毒株gag和env基因的亚型及序列分析]

[Subtype and sequence analysis of gag and env genes among HIV-1 strains circulating in Beijing residents during 2006].

作者信息

Ye Jing-rong, Xing Hui, Liu Hai-lin, Hei Fa-xin, Zhao Yue-juan, Liu Sheng-ya, Sun Wei-dong, Zhang Qi-yun, Zhang Qin, Lu Hong-yan, He Xiong, Shao Yi-ming

机构信息

Beijing Center for Disease Control and Prevention, Beijing 100013, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Jun;28(6):586-8.

PMID:17939390

Abstract

OBJECTIVE

To investigate the subtype distribution and the prevalence of sequence characteristics of HIV-1 strains in Beijing residents during 2006 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routines.

METHODS

Blood samples from 32 new confirmed HIV-1 infected individuals from Beijing residents in 2006 and separated plasma specimens were collected. RNAs were extracted and the gag and env gene were amplified by RT-PCR and nest-PCR. PCR products were sequenced directly and phylogenetic analyses of gag and env gene were performed using the MEGA2 software.

RESULTS

Among 32 HIV-1 plasma samples, 22 gag and 4 env gene fragments were amplified and analyzed. Five HIV-1 subtypes or circulating recombinant forms(CRFs) of HIV-1 including Thai B (2 strains), B (9 strains), C (2 strains), CRF07_BC (5 strains), CRF01 AE (4 strains) were identified being circulated in Beijing. The gene divergences of gag gene inside the subtypes were 6.6%, 4.3%, 6.8%, 4.9% and 3.0% in subtype B, Thai B, C, CRF01_AE and CRF07_BC respectively. Subtypes B were predominant in Beijing, accounted for 40.9% among 22 samples.

CONCLUSION

Five HIV-1 subtypes were identified in Beijing and the surveillance of HIV-1 gene variation should be paid more attention to.

摘要

目的

调查2006年北京市居民中HIV-1毒株的亚型分布及序列特征流行情况，并分析HIV-1亚型分布与传播途径之间的关系。

方法

收集2006年北京市32名新确诊的HIV-1感染居民的血样及分离的血浆标本。提取RNA，通过逆转录聚合酶链反应（RT-PCR）和巢式聚合酶链反应（nest-PCR）扩增gag和env基因。对PCR产物进行直接测序，并使用MEGA2软件对gag和env基因进行系统发育分析。

结果

在32份HIV-1血浆样本中，扩增并分析了22个gag基因片段和4个env基因片段。鉴定出5种HIV-1亚型或HIV-1的循环重组形式（CRF），包括泰国B亚型（2株）、B亚型（9株）、C亚型（2株）、CRF07_BC（5株）、CRF01_AE（4株）在北京流行。B亚型、泰国B亚型、C亚型、CRF01_AE亚型和CRF07_BC亚型内部gag基因的基因差异分别为6.6%、4.3%、6.8%、4.9%和3.0%。B亚型在北京占主导地位，在22份样本中占40.9%。