[重组人免疫缺陷病毒1型CRF01_AE毒株在中国的分布及其env基因V3-C3区的序列变异]
[Distribution of recombinant human immunodeficiency virus type-1 CRF01_AE strains in China and its sequence variations in the env V3-C3 region].
作者信息
Xing Hui, Liang Hao, Wan Zhuo-Yue, Chen Xi, Wei Min, Ma Peng-Fei, Guan Qi, Quan Yu, Hong Kun-Xue, Shao Yi-Ming
机构信息
National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2004 Sep;38(5):300-4.
OBJECTIVE
To characterize CRF01_AE strains of recombinant human immunodeficiency virus type-1 (HIV-1) found in the Second National Molecular Epidemiology Study on HIV in China and to analyze its sequence variation in the env V3-C3 region during the First National Molecular Epidemiology Study (NMES1, 1996 - 1998) to the Second National Molecular Epidemiology Study (NMES2, 2001 - 2002).
METHODS
DNA was extracted from peripheal blood mononuclear cells of the subjects with HIV infection. The env C2-V4 region of HIV-1 was amplified with nested polymerase chain reaction (n-PCR). PCR products were directly sequenced using ABI 377 DNA sequencer, then the gene-based phylogenetic tree was constructed and its variation of amino acids was analyzed with GCG software.
RESULTS
Totally, 169 strains of recombinant HIV-1 CRF01_AE were identified from blood samples collected from different high risk groups in 17 of 31 provinces, municipalities and autonomous regions all over China by the end of 2002. Although sexual transmission still dominated during NMES1 (62.2%, 23/37) and NMES2 (55.3%, 73/132), prevalence of HIV-1 CRF01_AE in intravenous drug users (IDUs) increased to 41.6% (57/137) during NMES2 from 27% (10/37) during NMES1. Phylogenetic tree analysis showed that HIV-1 CRF01_AE strains prevalent in IDUs during NMES2 did not cluster with those prevalent in the subjects infected by sexual transmission during NMES2 and those in IDUs during NMES1. The amino acid residues of V3 region of HIV-1 CRF01_AE in IDUs were relatively conservative, but the sixth, eighth, ninth, tenth, twelfth, fifteenth, sixteenth amino acid residues of C3 region displayed regular changes.
CONCLUSIONS
HIV-1 CRF01_AE strain has been introduced into inland provinces from southeastern coast areas and southwestern border areas, with an increasing prevalence in IDUs. The sequence of env V3-C3 region of recombinant HIV-1 CRF01_AE strains prevalent in IDUs during NMES2 was obviously different from that during NMES1, suggesting that HIV-1 CRF01_AE strains prevalent in IDUs during NMES2 might come from a new source and have a potential to spread.
目的
对中国第二次全国艾滋病分子流行病学研究中发现的重组1型人类免疫缺陷病毒（HIV-1）CRF01_AE毒株进行特征分析，并分析其在env V3-C3区从第一次全国艾滋病分子流行病学研究（NMES1，1996 - 1998年）到第二次全国艾滋病分子流行病学研究（NMES2，2001 - 2002年）期间的序列变异情况。
方法
从HIV感染受试者的外周血单个核细胞中提取DNA。采用巢式聚合酶链反应（n-PCR）扩增HIV-1的env C2-V4区。PCR产物用ABI 377 DNA测序仪直接测序，然后构建基于基因的系统发育树，并用GCG软件分析其氨基酸变异情况。
结果
截至2002年底，在中国31个省、直辖市和自治区中的17个地区，从不同高危人群采集的血样中总共鉴定出169株重组HIV-1 CRF01_AE毒株。虽然在NMES1（62.2%，23/37）和NMES2（55.3%，73/132）期间性传播仍占主导，但HIV-1 CRF01_AE在静脉吸毒者（IDU）中的流行率从NMES1期间的27%（10/37）增至NMES2期间的41.6%（57/137）。系统发育树分析显示，NMES2期间IDU中流行的HIV-1 CRF01_AE毒株与NMES2期间性传播感染受试者中流行的毒株以及NMES1期间IDU中流行的毒株未聚类。IDU中HIV-1 CRF01_AE的V3区氨基酸残基相对保守，但C3区的第六、八、九、十、十二、十五、十六位氨基酸残基呈现规律性变化。
结论
HIV-1 CRF01_AE毒株已从东南沿海地区和西南边境地区传入内陆省份，在IDU中的流行率呈上升趋势。NMES2期间IDU中流行的重组HIV-1 CRF01_AE毒株env V3-C3区序列与NMES1期间明显不同，提示NMES2期间IDU中流行的HIV-1 CRF01_AE毒株可能来自新的来源且有传播潜力。
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