Ye Jing-Rong, Li Yang, Bai Li-Shi
Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Bing Du Xue Bao. 2009 Nov;25(6):420-3.
To investigate the subtype distribution and sequence characteristics of HIV-1 strains prevalent in Beijing during 2007 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routes, we collected the anti-conglutinated whole blood samples from HIV-1 newly infected individuals in Beijing during 2007 and separated plasma specimens from the aamples. RNAs were extracted and the gag genes from the various samples were amplified by RT-PCR and nest-PCR. The PCR products were sequenced directly and phylogenetic analyses of gag genes were performed using the MEGA2 software. Among 200 HIV-1 plasma samples,161 gag HIV-1 gene fragments were amplified and analyzed. Seven HIV-1 subtypes or circulating recombinant forms (CRFs) of HIV-1 including A1 (1 strains), B (35 strains), Thai B (19 strains), C (3 strains), CRF01_AE (49 strains), CRF07_BC (51 strains), CRF08_BC (3 strain) were identified circulating in Beijing. The gene divergences inside the subtypes were different, with 7.7%, 6.5%, 6.7%, 6.7%, 5.5%, 4.3%, 5.8%, in subtype A1, B, Thai B, C, CRF01_AE, CRF07_BC, CRF08_BC, respectively. Subtypes CRF07_BC and CRF01_AE were predominant in Beijing account for 31.7% and 30.4% among samples. Seven HIV-1 subtypes exist in Beijing and the surveillance of HIV-1 gene variation should be paid more attention.
为研究2007年北京市流行的HIV-1毒株的亚型分布及序列特征，并分析HIV-1亚型分布与传播途径之间的关系，我们收集了2007年北京市HIV-1新感染者的抗凝血全血样本，并从样本中分离出血浆标本。提取RNA，通过逆转录聚合酶链反应（RT-PCR）和巢式聚合酶链反应（nest-PCR）扩增各样本的gag基因。对PCR产物进行直接测序，并使用MEGA2软件对gag基因进行系统发育分析。在200份HIV-1血浆样本中，扩增并分析了161个gag HIV-1基因片段。鉴定出7种HIV-1亚型或HIV-1的循环重组形式（CRF）在北京流行，包括A1（1株）、B（35株）、泰国B（19株）、C（3株）、CRF01_AE（49株）、CRF07_BC（51株）、CRF08_BC（3株）。各亚型内部的基因差异不同，A1、B、泰国B、C、CRF01_AE、CRF07_BC、CRF08_BC亚型的基因差异分别为7.7%、6.5%、6.7%、6.7%、5.5%、4.3%、5.8%。CRF07_BC和CRF01_AE亚型在北京占主导地位，在样本中分别占31.7%和30.4%。北京存在7种HIV-1亚型，应更加重视HIV-1基因变异的监测。
