García-Cañas Virginia, Cifuentes Alejandro
Institute of Industrial Fermentations, Madrid, Spain.
Electrophoresis. 2007 Nov;28(22):4013-30. doi: 10.1002/elps.200700253.
This work reviews the different analytical strategies based on capillary electromigration techniques developed for detecting microbial contaminants and their products in food matrices. Namely, this work presents an exhaustive and critical review, including works published till March 2007, on CE methods developed to detect and identify contaminants of microbial origin that represent a hazard to humans in foods. First, an overview on the strategies adopted for the analysis of intact microorganisms is presented. Next, CE methodologies based on the analysis of microbial constituents, including those based on genomics and proteomics approaches, are described. Finally, CE methods developed for detecting microbial toxins are discussed.
这项工作综述了基于毛细管电迁移技术开发的不同分析策略,这些技术用于检测食品基质中的微生物污染物及其产物。具体而言,这项工作对截至2007年3月发表的关于毛细管电泳(CE)方法的研究进行了详尽且批判性的综述,这些方法用于检测和识别食品中对人类具有危害的微生物源污染物。首先,介绍了用于分析完整微生物的策略概述。接下来,描述了基于微生物成分分析的CE方法,包括基于基因组学和蛋白质组学方法的那些。最后,讨论了用于检测微生物毒素的CE方法。