Suzurikawa Jun, Takahashi Hirokazu, Takayama Yuzo, Warisawa Shin'ichi, Mitsuishi Mamoru, Nakao Masayuki, Jimbo Yasuhiko
Dept. of Mechano-Informatics, The University of Tokyo, Japan.
Conf Proc IEEE Eng Med Biol Soc. 2006;2006:648-51. doi: 10.1109/IEMBS.2006.259828.
Conventional multielectrode arrays (MEAs) cannot always access desired neurons due to low electrode density and small number of electrode. To overcome this problem, we propose a light-addressable planar electrode on a glass substrate. The electrode has a 3-layer structure, namely a transparent SnO2 layer, an hydrogenated amorphous silicon (a-Si:H) layer, and a passivation layer. Illumination to the a-Si:H layer increases the conductivity of a-Si:H and creates a virtual electrode at the surface of the illuminated site. In the present study, we developed a low-conductive zinc antimonate-dispersed epoxy layer. This layer could successfully prevent penetration of culture medium and thus deterioration of a-Si:H layer. A fluo-4 calcium imaging demonstrated that, when the whole area of electrode was illuminated, negative-monophasic voltage-controlled pulses could also successfully activate neurons cultured on the electrode. Moreover, the focused illumination to the electrode resulted in the selective activation of neurons around the illuminated area.
传统的多电极阵列（MEA）由于电极密度低和电极数量少，无法总是接触到所需的神经元。为了克服这个问题，我们在玻璃基板上提出了一种光寻址平面电极。该电极具有三层结构，即透明的SnO2层、氢化非晶硅（a-Si:H）层和钝化层。对a-Si:H层进行光照会增加a-Si:H的导电性，并在光照部位的表面产生一个虚拟电极。在本研究中，我们开发了一种低导电性的锑酸锌分散环氧树脂层。该层可以成功地防止培养基渗透，从而防止a-Si:H层变质。Fluo-4钙成像显示，当电极的整个区域被照亮时，负单相电压控制脉冲也能成功激活培养在电极上的神经元。此外，对电极的聚焦光照导致了光照区域周围神经元的选择性激活。
