Reĭzin F N, Roĭkhel' V M, Chumakov M P
Antibiotiki. 1976 Jan;21(1):45-8.
Interferonogenesis in the cells of chick embryos induced by strain Lee of influenza B virus was studied. The levels of interferon production with and without changing the medium before the cell inoculation with the interferonogens were compared. It was shown that preliminary changing of the medium resulted in a statistically reliable increase in production of interferon as compared to that without the medium changing. It was found that the increase in the synthesis of interferon under the conditions of the maintaining medium changing was accompanied by an increase in the levels of synthesis of the cellular protein and RNA determined by incorporation of C14-leucine and H3-uridine into the acid insoluble fraction of the cells. It is concluded that metabolic changes in the cells at the moment of interferonogenesis induction due to the maintaining medium changing should be considered in estimation of the experiments on the mechanism of the virus induced interferonogenesis in the tissue culture.
研究了乙型流感病毒李株诱导鸡胚细胞产生干扰素的过程。比较了在接种干扰素原之前更换培养基和不更换培养基时干扰素的产生水平。结果表明，与不更换培养基相比，预先更换培养基可使干扰素产量在统计学上有显著增加。发现在维持培养基更换的条件下，干扰素合成的增加伴随着细胞蛋白质和RNA合成水平的增加，这是通过将C14-亮氨酸和H3-尿苷掺入细胞的酸不溶性部分来测定的。结论是，在评估组织培养中病毒诱导干扰素产生机制的实验时，应考虑由于维持培养基更换而在干扰素产生诱导时刻细胞中的代谢变化。
