Reizin F N, Roikhel V M, Chumakov M P
Arch Virol. 1975;49(4):307-15. doi: 10.1007/BF01318239.
The influence of cyclic 3'5'-adenosine monophosphate (cAMP), adrenalin and theophylline on interferon synthesis induced by influenza B virus (Lee strain) in chick embryo cell cultures was studied. In 5-day-old cell culture, theophylline was shown to enhance the inhibiting effect of exogenous cAMP and adrenalin on interferon synthesis and in 1-day-old culture, on the contrary, to enhance interferon production whereas adrenaline under these conditions had no effect on interferon synthesis at all. In 5-day-old cultures the activity of adrenalin and theophylline was manifested when they were added to the maintenance medium not later than 4 hours postinfection, and was not associated with the influence on interferon inducer adsorption on to cells or on virus multiplication in sensitive systems. Treatment of cells with these substances had no effect on interferon release from the cells. In the concentration used, adrenalin and theophylline exerted no cytotoxic effect. Theophylline inhibited incorporation of 3H-uridine and 14C-leucine into the acid insoluble fraction of the infected cells in 1-day-old cultures, while in 5-day-old cultures this was observed only when adrenalin and theophylline were used together. It is suggested that endogenous cAMP is essential for control of interferon synthesis and that different cAMP levels in cells of different ages may be one of the causes of the varying potency for interferon synthesis in young and old cell cultures.
研究了环磷腺苷（cAMP）、肾上腺素和茶碱对鸡胚细胞培养物中乙型流感病毒（Lee株）诱导的干扰素合成的影响。在5日龄细胞培养物中，茶碱显示可增强外源性cAMP和肾上腺素对干扰素合成的抑制作用；而在1日龄培养物中，情况则相反，茶碱可增强干扰素的产生，而在这些条件下肾上腺素对干扰素合成完全没有影响。在5日龄培养物中，肾上腺素和茶碱在感染后不迟于4小时添加到维持培养基中时表现出活性，且与它们对干扰素诱导剂吸附到细胞上或对敏感系统中病毒增殖的影响无关。用这些物质处理细胞对干扰素从细胞中的释放没有影响。在所使用的浓度下，肾上腺素和茶碱没有细胞毒性作用。茶碱在1日龄培养物中抑制3H-尿苷和14C-亮氨酸掺入感染细胞的酸不溶性部分，而在5日龄培养物中，只有当肾上腺素和茶碱一起使用时才会观察到这种情况。提示内源性cAMP对干扰素合成的控制至关重要，不同年龄细胞中不同的cAMP水平可能是年轻和年老细胞培养物中干扰素合成能力不同的原因之一。
