Kannan Lakshmi, Rath Narayan C, Liyanage Rohana, Lay Jackson O
Agricultural Research Service, USDA, Poultry Science Center, University of Arkansas, Fayetteville, AR 72701, USA.
Ann N Y Acad Sci. 2007 Sep;1112:425-34. doi: 10.1196/annals.1415.028.
The aim of the study was to determine chicken monocyte- and granulocyte-associated peptides and proteins using "whole cell" matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and to characterize the peptides based on their abundance. The mass spectra showed a prominent peak at m/z 4963 in monocytes/macrophages but not in the granulocytes. Subsequent purification and characterization of the m/z 4963 peptide from an avian macrophage cell line HTC, revealed it to be thymosin beta-4 (Tbeta-4), an actin-modulating peptide. HTC cells when treated with bacterial lipopolysaccharide and peptidoglycan to determine the modulation of Tbeta-4 gene expression or its secretion, showed no changes.
本研究的目的是使用“全细胞”基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）来确定鸡单核细胞和粒细胞相关的肽和蛋白质，并根据其丰度对肽进行表征。质谱显示，单核细胞/巨噬细胞中在m/z 4963处有一个突出的峰，而粒细胞中没有。随后从禽巨噬细胞系HTC中对m/z 4963肽进行纯化和表征，发现它是胸腺素β-4（Tbeta-4），一种肌动蛋白调节肽。用细菌脂多糖和肽聚糖处理HTC细胞以确定Tbeta-4基因表达或其分泌的调节情况，结果显示没有变化。
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