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使用毛细管电泳、高效液相色谱、基质辅助激光解吸电离和埃德曼降解法对牛组织中的天然肽进行表征。

Characterization of natural peptides from bovine tissue using capillary electrophoresis, high performance liquid chromatography, matrix-assisted laser desorption ionization, and Edman degradation.

作者信息

Weiler A, Stoeva S, Lehmann R, Grübler G, Paulus G, Voelter W

机构信息

Abteilung für Physikalische Biochemie, Universität Tübingen, Germany.

出版信息

Electrophoresis. 1996 Mar;17(3):518-22. doi: 10.1002/elps.1150170317.

DOI:10.1002/elps.1150170317
PMID:8740171
Abstract

A combination of high performance liquid chromatography (HPLC) and capillary electrophoresis (CE) spiking, Edman degradation, amino acid analysis and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) was applied for the analysis of the primary structure of beta-thymosins. CE has been used to resolve peaks which coelute on HPLC, as well as to help establish the identity of tryptic fragments in the peptide mapping experiments of the two highly homologous beta-thymosins.

摘要

高效液相色谱（HPLC）、毛细管电泳（CE）加样、埃德曼降解法、氨基酸分析和基质辅助激光解吸电离质谱（MALDI-MS）相结合，用于分析β-胸腺素的一级结构。CE已被用于分离在HPLC上共洗脱的峰，以及在两种高度同源的β-胸腺素的肽图谱实验中帮助确定胰蛋白酶片段的身份。

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