基于巢式PCR技术检测韩国德国牧羊犬和流浪猫中的弓形虫
Nested PCR-based detection of Toxoplasma gondii in German shepherd dogs and stray cats in South Korea.
作者信息
Lee J Y, Lee S E, Lee E G, Song K H
机构信息
Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, South Korea.
出版信息
Res Vet Sci. 2008 Aug;85(1):125-7. doi: 10.1016/j.rvsc.2007.09.006. Epub 2007 Oct 18.
The prevalence of Toxoplasma gondii was surveyed by using a nested polymerase chain reaction (PCR) that was targeted to T. gondii B1 gene in German shepherd dogs and stray cats. Sixty-four (46.3%) out of 138 German shepherd dogs and 50 (47.2%) out of 106 stray cats were tested positive by the nested PCR assay, respectively. There was no significant difference in gender or age in German shepherd dogs and stray cats. In the five positive dogs and five positive cats, the nucleotide partial sequence of the T. gondii B1 gene was identified by direct sequence analysis. All the sequences were identical to each other and the corresponding sequence, T. gondii B1 gene (Accession No. AF179871). The results suggest that the prevalence of T. gondii is high, and the nested PCR assay is useful for early detection of T. gondii for asymptomatic dogs and cats.
采用针对弓形虫B1基因的巢式聚合酶链反应（PCR），对德国牧羊犬和流浪猫进行弓形虫感染率调查。138只德国牧羊犬中有64只（46.3%）、106只流浪猫中有50只（47.2%）通过巢式PCR检测呈阳性。德国牧羊犬和流浪猫在性别或年龄上无显著差异。在5只阳性犬和5只阳性猫中，通过直接序列分析鉴定了弓形虫B1基因的核苷酸部分序列。所有序列彼此相同，且与相应序列——弓形虫B1基因（登录号AF179871）相同。结果表明，弓形虫感染率很高，巢式PCR检测对于无症状犬猫的弓形虫早期检测很有用。
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