Park Yeojin, Noh Jinhyeong, Seo Hyun-Ji, Kim Keun-Ho, Min Subin, Yoo Mi-Sun, Yun Bo-Ram, Kim Jong-Ho, Choi Eun-Jin, Cheon Doo-Sung, Hong Sung-Jong, Yoon Soon-Seek, Cho Yun Sang
Parasitic and Honeybee Disease Laboratory, Bacterial and Parasitic Disease Division, Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Animal Pathodiagnostic Laboratory, Animal Disease Diagnostic Division, Department of Disease Control & Quarantine, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Korean J Parasitol. 2020 Jun;58(3):257-265. doi: 10.3347/kjp.2020.58.3.257. Epub 2020 Jun 26.
The outbreak of human toxoplasmosis can be attributed to ingestion of food contaminated with Toxoplasma gondii. Toxoplasmosis recently increased in domestic and stray dogs and cats. It prompted studies on the zoonotic infectious diseases transmitted via these animals. Sero- and antigen prevalences of T. gondii in dogs and cats were surveyed using ELISA and PCR, and B1 gene phylogeny was analyzed in this study. Toxoplasmosis antibodies were measured on sera of 403 stray cats, 947 stray dogs, 909 domestic cats, and 2,412 domestic dogs collected at nationwide regions, Korea from 2017 to 2019. In addition, whole blood, feces, and tissue samples were also collected from stray cats (1,392), stray dogs (686), domestic cats (3,040), and domestic dogs (1,974), and T. gondii-specific B1 gene PCR was performed. Antibody prevalence of stray cats, stray dogs, domestic cats, and domestic dogs were 14.1%, 5.6%, 2.3%, and 0.04%, respectively. Antigen prevalence of these animals was 0.5%, 0.2%, 0.1%, and 0.4%, respectively. Stray cats revealed the highest infection rate of toxoplasmosis, followed by stray dogs, domestic cats, and domestic dogs. B1 gene positives were 5 of stray cats, and identified to high/moderate pathogenic Type I/III group. These findings enforce that preventive hygienic measure should be strengthened at One Health level in dogs and cats, domestic and stray, to minimize human toxoplasmosis infections.
人类弓形虫病的爆发可归因于摄入被刚地弓形虫污染的食物。最近，家犬和流浪猫狗的弓形虫病有所增加。这促使人们对通过这些动物传播的人畜共患传染病进行研究。本研究采用酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）对猫狗体内弓形虫的血清学和抗原流行率进行了调查，并分析了B1基因系统发育。对2017年至2019年在韩国全国各地区采集的403只流浪猫、947只流浪狗、909只家猫和2412只家犬的血清进行了弓形虫病抗体检测。此外，还从流浪猫（1392只）、流浪狗（686只）、家猫（3040只）和家犬（1974只）采集了全血、粪便和组织样本，并进行了弓形虫特异性B1基因PCR检测。流浪猫、流浪狗、家猫和家犬的抗体流行率分别为14.1%、5.6%、2.3%和0.04%。这些动物的抗原流行率分别为0.5%、0.2%、0.1%和0.4%。流浪猫的弓形虫感染率最高，其次是流浪狗、家猫和家犬。B1基因阳性的流浪猫有5只，被鉴定为高/中度致病性I/III型组。这些发现强调，应在“同一健康”层面加强对家养和流浪猫狗的预防性卫生措施，以尽量减少人类弓形虫病感染。
