Dominguez-Steglich M, Auffray C, Schmid M
Department of Human Genetics, University of Würzburg, Germany.
J Hered. 1991 Nov-Dec;82(6):503-5. doi: 10.1093/oxfordjournals.jhered.a111138.
Linkage of the chicken major histocompatibility complex (B-complex) to the nucleolus organizer regions (NOR) has been confirmed in situ. Nonradioactive hybridization to metaphase chromosomes using a pool of biotinylated cDNA probes for the B-F, B-L beta, and B-G genes of the B-complex gave positive signals on one or a pair of microchromosomes. Subsequent staining with silver nitrate proved these microchromosomes to be the NOR-bearing pair. The value of employing nonisotopic in situ hybridization techniques for mapping of genes and gene complexes, especially when dealing with microchromosomes, is stressed.
鸡主要组织相容性复合体（B复合体）与核仁组织区（NOR）的连锁关系已在原位得到证实。使用针对B复合体的B-F、B-Lβ和B-G基因的生物素化cDNA探针池与中期染色体进行非放射性杂交，在一条或一对微染色体上产生了阳性信号。随后用硝酸银染色证明这些微染色体是携带NOR的一对染色体。强调了采用非同位素原位杂交技术进行基因和基因复合体定位的价值，特别是在处理微染色体时。
