Miller M M, Goto R, Bernot A, Zoorob R, Auffray C, Bumstead N, Briles W E
Department of Molecular Biochemistry, Beckman Research Institute of the City of Hope, Duarte, CA 91001-0269.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4397-401. doi: 10.1073/pnas.91.10.4397.
Gene sequences highly similar to major histocompatibility complex (Mhc) class I and class II genes were recently recognized as mapping to a site in the genome of the chicken separate from the Mhc class I, class II, and B-G genes of the major histocompatibility (B) complex. The present study was undertaken to see whether this complex of Mhc-like genes designated as restriction fragment pattern Y (Rfp-Y) might reside in one of three clusters of cosmid clones contained within the molecular map of chicken Mhc genes, since only two of the three clusters can be assigned to the B system. To determine whether the third cluster (cluster II/IV) might contain Rfp-Y, a subclone (18.1) from within cluster II/IV near a polymorphic lectin gene was used to analyze the DNA of families in which Rfp-Y haplotypes are known to be segregating. The restriction fragment polymorphisms revealed by the 18.1 probe were found to segregate in parallel with the restriction fragment polymorphisms defining the Rfp-Y haplotypes, thus establishing the location of Rfp-Y within cosmid cluster II/IV. Two of six Mhc class I genes and two of five Mhc class II genes map to cosmid cluster II/IV, so a substantial fraction of chicken Mhc genes, including at least one that may be expressed, are located in a chromosomal region separate from the B system. In further linkage analyses, Rfp-Y was found to assort independently from more than 400 markers in the present linkage map of the chicken genome.
与主要组织相容性复合体（Mhc）I类和II类基因高度相似的基因序列，最近被认定定位于鸡基因组中的一个位点，该位点与主要组织相容性（B）复合体的Mhc I类、II类和B - G基因分开。本研究旨在探究这种被指定为限制性片段模式Y（Rfp - Y）的Mhc样基因复合体，是否可能存在于鸡Mhc基因分子图谱中包含的三个黏粒克隆簇之一中，因为这三个簇中只有两个可归属于B系统。为了确定第三个簇（簇II/IV）是否可能包含Rfp - Y，使用了来自簇II/IV中靠近一个多态性凝集素基因的一个亚克隆（18.1），来分析已知Rfp - Y单倍型正在分离的家系的DNA。发现由18.1探针揭示的限制性片段多态性与定义Rfp - Y单倍型 的限制性片段多态性平行分离，从而确定了Rfp - Y在黏粒簇II/IV中的位置。六个Mhc I类基因中的两个和五个Mhc II类基因中的两个定位于黏粒簇II/IV，因此相当一部分鸡Mhc基因，包括至少一个可能表达的基因，位于与B系统分开的染色体区域。在进一步的连锁分析中，发现Rfp - Y与鸡基因组当前连锁图谱中的400多个标记独立分配。
