Evans Richard D, Itoh Yoshifumi
Methods Mol Med. 2007;135:239-49. doi: 10.1007/978-1-59745-401-8_15.
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a type I transmembrane protein which exhibits biological activities on the cell surface. One of the characteristic functions of this enzyme is activation of proMMP-2 on the cell surface (1). This process can be monitored using gelatin zymography to detect the pro- and active forms of MMP-2. Cellular MT1-MMP activity can also be directly detected by in situ degradation of layers of either gelatin or collagen. By combining inhibitors, western blotting, and immunohistochemistry, one can detect and conclude the presence of MT1-MMP activity in the cells.
膜型1基质金属蛋白酶（MT1-MMP）是一种I型跨膜蛋白，在细胞表面发挥生物学活性。该酶的一个特征性功能是在细胞表面激活前MMP-2（1）。此过程可通过明胶酶谱法监测，以检测MMP-2的前体形式和活性形式。细胞MT1-MMP活性也可通过明胶或胶原蛋白层的原位降解直接检测。通过结合抑制剂、蛋白质印迹法和免疫组织化学，可检测并确定细胞中MT1-MMP活性的存在。
