Szekanecz Zoltán, Koch Alisa E
Methods Mol Med. 2007;135:359-64. doi: 10.1007/978-1-59745-401-8_23.
The ingress of leukocytes into the synovium is a key event in inflammatory arthritis, such as rheumatoid arthritis. A number of soluble inflammatory mediators, such as chemokines, cytokines, and soluble adhesion molecules are involved in this event. It is feasible to test the chemotactic activity of mononuclear cells or endothelial cells using in vitro chemotaxis assays. The role of various chemotactic mediators in inflammatory synovitis can also be assessed using these systems by including these agents in the assay. There we describe monocyte and endothelial cell chemotaxis assays used in our laboratory.
白细胞进入滑膜是炎症性关节炎（如类风湿性关节炎）中的关键事件。多种可溶性炎症介质，如趋化因子、细胞因子和可溶性黏附分子都参与了这一过程。利用体外趋化试验检测单核细胞或内皮细胞的趋化活性是可行的。通过在试验中加入这些介质，还可以利用这些系统评估各种趋化介质在炎症性滑膜炎中的作用。在此，我们描述了我们实验室使用的单核细胞和内皮细胞趋化试验。
