Department of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114-2790, USA.
Nucleic Acids Res. 2010 Jan;38(Database issue):D792-9. doi: 10.1093/nar/gkp1005. Epub 2009 Nov 11.
PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26,855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.
PrimerBank（http://pga.mgh.harvard.edu/primerbank/）是一个公共资源，用于通过 PCR 和定量 PCR（QPCR）检索人类和小鼠基因表达分析的引物对。从 PrimerBank 数据库中可以访问总共 306,800 个覆盖大多数已知人类和小鼠基因的引物，以及有关这些引物的信息，例如 T(m)、转录本上的位置和扩增子大小。对于每个基因，至少设计了一对引物，在许多情况下存在替代引物对。已经设计了引物以在相同的 PCR 条件下工作，从而便于高通量 QPCR。有几种方法可以搜索感兴趣的基因的引物，例如：GenBank 访问号、NCBI 蛋白质访问号、NCBI 基因 ID、PrimerBank ID、NCBI 基因符号或基因描述（关键字）。总共，已经通过 QPCR、琼脂糖凝胶分析、测序和 BLAST 实验验证了 26,855 对覆盖大多数已知小鼠基因的引物，所有验证数据均可从 PrimerBank 网站免费访问。
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