Wedemeyer Christian, Xu Jie, Neuerburg Carl, Landgraeber Stefan, Malyar Nasser M, von Knoch Fabian, Gosheger Georg, von Knoch Marius, Löer Franz, Saxler Guido
Department of Orthopedics, University of Duisburg-Essen, Hufelandstr. 55, 45122 Essen, Germany.
Calcif Tissue Int. 2007 Nov;81(5):394-402. doi: 10.1007/s00223-007-9077-2. Epub 2007 Oct 21.
Small-animal models are useful for the in vivo study of particle-induced osteolysis, the most frequent cause of aseptic loosening after total joint replacement. Microstructural changes associated with particle-induced osteolysis have been extensively explored using two-dimensional (2D) techniques. However, relatively little is known regarding the 3D dynamic microstructure of particle-induced osteolysis. Therefore, we tested micro-computed tomography (micro-CT) as a novel tool for 3D analysis of wear debris-mediated osteolysis in a small-animal model of particle-induced osteolysis. The murine calvarial model based on polyethylene particles was utilized in 14 C57BL/J6 mice randomly divided into two groups. Group 1 received sham surgery, and group 2 was treated with polyethylene particles. We performed 3D micro-CT analysis and histological assessment. Various bone morphometric parameters were assessed. Regression was used to examine the relation between the results achieved by the two methods. Micro-CT analysis provides a fully automated means to quantify bone destruction in a mouse model of particle-induced osteolysis. This method revealed that the osteolytic lesions in calvaria in the experimental group were affected irregularly compared to the rather even distribution of osteolysis in the control group. This is an observation which would have been missed if histomorphometric analysis only had been performed, leading to false assessment of the actual situation. These irregularities seen by micro-CT analysis provide new insight into individual bone changes which might otherwise be overlooked by histological analysis and can be used as baseline information on which future studies can be designed.
小动物模型对于颗粒诱导的骨溶解的体内研究很有用，颗粒诱导的骨溶解是全关节置换术后无菌性松动最常见的原因。使用二维（2D）技术已广泛探索了与颗粒诱导的骨溶解相关的微观结构变化。然而，关于颗粒诱导的骨溶解的三维动态微观结构，人们了解得相对较少。因此，我们测试了微型计算机断层扫描（micro-CT）作为一种新型工具，用于在颗粒诱导的骨溶解的小动物模型中对磨损颗粒介导的骨溶解进行三维分析。基于聚乙烯颗粒的小鼠颅骨模型用于14只随机分为两组的C57BL/J6小鼠。第1组接受假手术，第2组用聚乙烯颗粒治疗。我们进行了三维micro-CT分析和组织学评估。评估了各种骨形态计量学参数。使用回归分析来检验两种方法所得结果之间的关系。Micro-CT分析提供了一种完全自动化的方法来量化颗粒诱导的骨溶解小鼠模型中的骨破坏。该方法显示，与对照组中较为均匀分布的骨溶解相比，实验组颅骨中的溶骨病变受到的影响不规则。如果仅进行组织形态计量学分析，这一观察结果将会被遗漏，从而导致对实际情况的错误评估。通过micro-CT分析看到的这些不规则情况为个体骨变化提供了新的见解，否则这些变化可能会被组织学分析忽略，并且可以用作设计未来研究的基线信息。
