Zhang Jun-zhe, Wang Jin-hong, Qin Yang, Fu Qiang
Department of Biochemistry and molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Sep;38(5):761-5.
To investigate the reactivation of hypermethylated GSTP1 (glutathione S-transferase P1) promoter activity by a component of natural drug, CDP.
The recombinant plasmid of pGL3-GSTP1 pro(m) containing hypermethylated GSTP1 gene promoter was constructed and then used to transiently transfect the human breast cancer cell line MCF-7 cell. After treatment with CDP and 5-aza-C, The luciferase activity in cell lysates were assayed.
Low promoter activities were found in hypermethylated GSTP1 promoter. The promoter activities were reactivated and in a CDP dose-dependent mode.
CDP has the ability to reactivate the hypermethylated GSTP1 gene promoter activity.
研究天然药物成分CDP对高甲基化的谷胱甘肽S-转移酶P1（GSTP1）启动子活性的再激活作用。
构建含有高甲基化GSTP1基因启动子的pGL3-GSTP1 pro(m)重组质粒，然后用于瞬时转染人乳腺癌细胞系MCF-7细胞。用CDP和5-氮杂胞苷处理后，测定细胞裂解物中的荧光素酶活性。
高甲基化的GSTP1启动子活性较低。启动子活性被再激活，且呈CDP剂量依赖性。
CDP具有再激活高甲基化GSTP1基因启动子活性的能力。
