三种环孢素治疗药物监测方法的比较。

Comparison of three methods for cyclosporine therapeutic monitoring.

作者信息

Jebabli N, Klouz A, Bahlous A, Gaïes E, Trabelsi S, Lakhal M, Belkahia C

机构信息

Centre National de Pharmacovigilance, Laboratoire de Pharmacologie Clinique, Hôpital Charles Nicolle, 9 Rue Dr Zouheir Assafi, 1006 Tunis, Tunisia.

出版信息

Transplant Proc. 2007 Oct;39(8):2557-9. doi: 10.1016/j.transproceed.2007.08.027.

DOI:10.1016/j.transproceed.2007.08.027

PMID:17954172

Abstract

Cyclosporine (CsA) is an immunosuppressive drug used extensively in human transplants of solid organs or bone marrow as well as in the treatment of autoimmune diseases. To optimize immunosuppressive efficacy and minimize adverse reactions, blood CsA concentrations are monitored to allow appropriate dosage adjustments. To establish objective criteria to compare various techniques of CsA monitoring, we performed a detailed study over 5 months to compare and evaluate three immunoassays methods in comparison to the reference method of high-performance liquid chromatography (HPLC). Our study included 976 samples that were evaluated by: the COBAS INTEGRA 800 (Roche Laboratories); the V-Twin (Dade Behring Laboratories); and the AxSYM FPIA (Abbott Laboratories). Our results showed that all of the immunoassays yielded slightly higher concentrations than HPLC. However CsA concentrations obtained by AxSYM were most close to those of HPLC, so that this method seemed to be more specific than the other two.

摘要

环孢素（CsA）是一种免疫抑制药物，广泛应用于人体实体器官或骨髓移植以及自身免疫性疾病的治疗。为了优化免疫抑制效果并将不良反应降至最低，需监测血液中环孢素的浓度以便进行适当的剂量调整。为了建立客观标准来比较环孢素监测的各种技术，我们进行了为期5个月的详细研究，以比较和评估三种免疫分析方法，并与高效液相色谱法（HPLC）的参考方法进行对比。我们的研究包括976个样本，这些样本通过以下方法进行评估：COBAS INTEGRA 800（罗氏实验室）；V-Twin（达德拜耳实验室）；以及AxSYM荧光偏振免疫分析（雅培实验室）。我们的结果表明，所有免疫分析方法得出的浓度均略高于HPLC法。然而，AxSYM法测得的环孢素浓度最接近HPLC法，因此该方法似乎比其他两种方法更具特异性。