Wang Dingzhi, DuBois Raymond N
Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
Methods Enzymol. 2007;433:27-50. doi: 10.1016/S0076-6879(07)33002-4.
Eicosanoids derived from arachidonic acid through cyclooxygenase (COX), lipoxygenase (LOX), and P450 pathways include prostanoids, hydroxyeicosatetraenoic acids (HETEs), leukotrienes (LTs), and epoxyeicosatrienoic acids (EETs). These bioactive lipids play an important role in regulating cell proliferation, apoptosis, tissue repair, blood clotting, blood vessel permeability, inflammation, and immune cell behavior. Moreover, some of these eicosanoids also modulate inflammation and tumor growth in cancer tissues, and may serve as biomarkers for monitoring colorectal cancer progression or as an intermediate marker for the pharmacologic activity of chemopreventive agents. Development of sensitive, rapid, and specific methods for determining eicosanoid levels accurately will facilitate an understanding of the biologic functions of these lipid mediators and will broaden our insight of the importance of these bioactive lipids in vivo. However, quantitative determination of eicosanoids in biological samples has presented a problem to many investigators. It is necessary to understand the advantages and limitations of each method for quantitative analysis of specific eicosanoids in various types of biological samples. Here we evaluate the methodology of the measurement of eicosanoids in biological samples.
通过环氧化酶（COX）、脂氧合酶（LOX）和细胞色素P450途径从花生四烯酸衍生而来的类二十烷酸包括前列腺素、羟基二十碳四烯酸（HETEs）、白三烯（LTs）和环氧二十碳三烯酸（EETs）。这些生物活性脂质在调节细胞增殖、凋亡、组织修复、血液凝固、血管通透性、炎症和免疫细胞行为方面发挥着重要作用。此外，其中一些类二十烷酸还调节癌症组织中的炎症和肿瘤生长，并可作为监测结直肠癌进展的生物标志物或作为化学预防剂药理活性的中间标志物。开发灵敏、快速且特异的方法以准确测定类二十烷酸水平，将有助于理解这些脂质介质的生物学功能，并拓宽我们对这些生物活性脂质在体内重要性的认识。然而，生物样品中类二十烷酸的定量测定给许多研究人员带来了难题。有必要了解各种生物样品中特定类二十烷酸定量分析方法的优缺点。在此，我们评估生物样品中类二十烷酸测量的方法学。
