Cancer Biology Group, Department of Endocrinology and Sydney Cancer Centre, Central Clinical School, The University of Sydney, NSW, Australia.
Free Radic Res. 2009 Oct;43(10):951-6. doi: 10.1080/10715760903145013. Epub 2009 Aug 12.
Arachidonic acid (AA) reaction with cyclooxygenase (COX) and lipoxygenases (LOX) yield eicosanoids that can mediate prostate cancer proliferation and enhance both tumour vascularization and metastasis. Increasingly measurement of eicosanoids with liquid chromatography is employed to implicate LOX activity in different biological systems and in particular link LOX activity to the progression of cancer in experimental models. This study demonstrates that simply identifying patterns of eicosanoid regio-isomerism is insufficient to designate LOX activity in prostate cancer cells and the analysis must include complete stereochemical assignment of the various isomers in order to validate the assignment of LOX activity.
花生四烯酸（AA）与环加氧酶（COX）和脂加氧酶（LOX）反应生成的类二十烷酸可介导前列腺癌增殖，并增强肿瘤血管生成和转移。越来越多地采用液相色谱法测量类二十烷酸，以表明 LOX 活性在不同的生物系统中，并特别将 LOX 活性与实验模型中的癌症进展联系起来。本研究表明，仅仅确定类二十烷酸区域异构体的模式不足以确定前列腺癌细胞中的 LOX 活性，分析必须包括各种异构体的完整立体化学赋值，以验证 LOX 活性的赋值。
