Wijesinghe Dayanjan S, Lamour Nadia F, Gomez-Munoz Antonio, Chalfant Charles E
Department of Biochemistry and Molecular Biology, Virginia Commonwealth University School of Medicine, Richmond, Virginia, USA.
Methods Enzymol. 2007;434:265-92. doi: 10.1016/S0076-6879(07)34015-9.
It has been over a decade since the sphingolipid ceramide-1-phosphate (C1P) was described. Until recently, only sparse reports on possible biological functions for this lipid have been published. A large number of reports have now surfaced demonstrating distinct biological mechanisms regulated by C1P produced from ceramide kinase (CERK). In the following methods chapter, the methodologies for examining CERK function in vitro and in cells are outlined in detail. The methodologies for examining C1P levels and the use of exogenous C1P on cells to observe lipid specific effects on a particular biology are also detailed.
自鞘脂神经酰胺-1-磷酸（C1P）被描述以来，已经过去了十多年。直到最近，关于这种脂质可能的生物学功能的报道还很稀少。现在大量的报道涌现出来，证明了由神经酰胺激酶（CERK）产生的C1P所调节的独特生物学机制。在接下来的方法章节中，将详细概述在体外和细胞中检测CERK功能的方法。还将详细介绍检测C1P水平的方法以及在细胞上使用外源性C1P来观察脂质对特定生物学的特异性影响。
