Yu Jia Ning, Huang Jian, Wang Zi Ning, Zhang Jin Song, Chen Shou Yi
National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
J Biosci. 2007 Sep;32(6):1153-61. doi: 10.1007/s12038-007-0117-x.
A vacuole Na+/H+ antiporter gene TaNHX2 was obtained by screening the wheat cDNA library and by the 5'-RACE method. The expression of TaNHX2 was induced in roots and leaves by treatment with NaCl, polyethylene glycol (PEG), cold and abscisic acid (ABA). When expressed in a yeast mutant (deltanhx1), TaNHX2 suppressed the salt sensitivity of the mutant,which was deficient in vacuolar Na+/H+ antiporter, and caused partial recovery of growth of delta nhx1 in NaCl and LiCl media. The survival rate of yeast cells was improved by overexpressing the TaNHX2 gene under NaCl, KCl, sorbitol and freezing stresses when compared with the control. The results imply that TaNHX2 might play an important role in salt and osmotic stress tolerance in plant cells.
通过筛选小麦cDNA文库并采用5'-RACE方法获得了一个液泡Na+/H+逆向转运蛋白基因TaNHX2。用NaCl、聚乙二醇（PEG）、低温和脱落酸（ABA）处理后，TaNHX2在根和叶中被诱导表达。当在酵母突变体（deltanhx1）中表达时，TaNHX2抑制了该突变体的盐敏感性，该突变体缺乏液泡Na+/H+逆向转运蛋白，并使deltanhx1在NaCl和LiCl培养基中的生长部分恢复。与对照相比，在NaCl、KCl、山梨醇和冷冻胁迫下过表达TaNHX2基因可提高酵母细胞的存活率。结果表明，TaNHX2可能在植物细胞的耐盐和渗透胁迫中发挥重要作用。
