Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University, Zhengzhou 450002, China.
Weinan Vocational and Technical College, Weinan 714026, China.
Int J Mol Sci. 2021 Apr 14;22(8):4047. doi: 10.3390/ijms22084047.
Vacuolar sodium/proton (Na/H) antiporters (NHXs) can stabilize ion contents to improve the salt tolerance of plants. Here, was cloned and characterized from upland cotton (). Phylogenetic and sequence analyses showed that belongs to the vacuolar-type NHXs. The GhNHX3D-enhanced green fluorescent protein (eGFP) fusion protein localized on the vacuolar membrane when transiently expressed in protoplasts. The quantitative real-time PCR (qRT-PCR) analysis showed that was induced rapidly in response to salt stress in cotton leaves, and its transcript levels increased with the aggravation of salt stress. The introduction of into the salt-sensitive yeast mutant ATX3 improved its salt tolerance. Furthermore, silencing of in cotton plants by virus-induced gene silencing (VIGS) increased the Na levels in the leaves, stems, and roots and decreased the K content in the roots, leading to greater salt sensitivity. Our results indicate that is a member of the vacuolar NHX family and can confer salt tolerance by adjusting the steady-state balance of cellular Na and K ions.
液泡钠离子/质子（Na/H）反向转运蛋白（NHXs）可以稳定离子含量，从而提高植物的耐盐性。本研究从陆地棉（）中克隆并鉴定了一个 NHX 基因（）。系统进化和序列分析表明，该基因属于液泡型 NHX。GhNHX3D-增强型绿色荧光蛋白（eGFP）融合蛋白在棉花原生质体中瞬时表达时定位于液泡膜上。实时定量 PCR（qRT-PCR）分析表明，GhNHX3D 在棉花叶片中受到盐胁迫的快速诱导，其转录水平随盐胁迫的加剧而增加。将GhNHX3D 导入盐敏感酵母突变体 ATX3 中提高了其耐盐性。此外，通过病毒诱导的基因沉默（VIGS）沉默棉花植株中的GhNHX3D 会导致叶片、茎和根中的 Na 水平升高，根中的 K 含量降低，从而增加盐敏感性。研究结果表明，GhNHX3D 是液泡 NHX 家族的一员，通过调节细胞内 Na 和 K 离子的稳态平衡赋予植物耐盐性。
