使用涂覆有过氧化聚吡咯的微电极阵列对脑组织中的细胞外谷氨酸进行选择性检测。
Selective detection of extracellular glutamate in brain tissue using microelectrode arrays coated with over-oxidized polypyrrole.
作者信息
Walker Eric, Wang Jianjun, Hamdi Naser, Monbouquette Harold G, Maidment Nigel T
机构信息
Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience & Human Behavior, University of California, Los Angeles, CA 90095, USA.
出版信息
Analyst. 2007 Nov;132(11):1107-11. doi: 10.1039/b706880h. Epub 2007 Sep 19.
We describe the selective measurement of extracellular glutamate concentration in rodent brain using ceramic-based platinum microelectrode arrays (MEAs) coated with electropolymerized, over-oxidized polypyrrole (OPP) as a permselective barrier to interference from dopamine and ascorbate. OPP-coated MEAs displayed similar sensitivity (97 +/- 9 nA microM(-1) cm(-2)) and response time (ca. 1 s) to glutamate as previously described Nafion-coated MEAs but, unlike Nafion-coated MEAs, were unresponsive to 5 microM dopamine. Such OPP-coated MEAs afforded selective detection of evoked glutamate release in the dopamine-rich striatum of the mouse brain.
我们描述了使用涂覆有经电聚合的过氧化聚吡咯（OPP）的陶瓷基铂微电极阵列（MEA）对啮齿动物大脑中的细胞外谷氨酸浓度进行选择性测量，该聚吡咯作为一种选择性渗透屏障，可防止多巴胺和抗坏血酸的干扰。与先前描述的涂覆有Nafion的MEA相比，涂覆有OPP的MEA对谷氨酸显示出相似的灵敏度（97±9 nA μM⁻¹ cm⁻²）和响应时间（约1秒），但与涂覆有Nafion的MEA不同，对5 μM多巴胺无反应。这种涂覆有OPP的MEA能够在富含多巴胺的小鼠大脑纹状体中选择性检测诱发的谷氨酸释放。
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