Soldatkina O V, Soldatkin O O, Kasap B Ozansoy, Kucherenko D Yu, Kucherenko I S, Kurc B Akata, Dzyadevych S V
Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, Kyiv, 01003, Ukraine.
Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, Kyiv, 03143, Ukraine.
Nanoscale Res Lett. 2017 Dec;12(1):260. doi: 10.1186/s11671-017-2026-8. Epub 2017 Apr 7.
In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.
在这项工作中，我们开发了一种用于检测谷氨酸的新型安培生物传感器，采用通过吸附在硅沸石颗粒上固定谷氨酸氧化酶（GlOx）的典型方法。圆盘铂电极（直径 = 0.4 毫米）用作安培传感器。对生物传感器的制备过程进行了优化。确定了用硅沸石层修饰安培换能器的主要参数以及GlOx在该层上的吸附过程。基于吸附在硅沸石上的GlOx的生物传感器对谷氨酸表现出高灵敏度。检测的线性范围为2.5至450μM，谷氨酸的检测限为1μM。结果表明，所提出的生物传感器在连续工作数小时期间具有良好的响应重现性，并且在几天内具有操作稳定性。所开发的生物传感器可用于实际样品中谷氨酸的测定。
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