Dallo S F, Baseman J B
Department of Microbiology, University of Texas Health Science Center, San Antonio 78254-7758.
Microb Pathog. 1991 Jun;10(6):475-80. doi: 10.1016/0882-4010(91)90113-o.
The structural gene of the 140 kDa adhesin of Mycoplasma genitalium was used to probe M. genitalium genomic DNA for gene copy number. Since multiple banding patterns were observed, the 140 kDa structural gene was subdivided into 10 contiguous fragments in size from 165 to 657 base pairs in order to determine which parts of the adhesin gene existed as multiple copies. Each fragment was labeled by nick translation and used to probe the entire M. genitalium genomic DNA. Approximately half the gene was present in single copy while the remaining sequences were multiple copied. Both single and multiple copy regions were interspersed throughout the structural gene.
利用生殖支原体140 kDa黏附素的结构基因探测生殖支原体基因组DNA的基因拷贝数。由于观察到多种条带模式，将140 kDa结构基因细分为10个连续片段，大小从165至657个碱基对，以确定黏附素基因的哪些部分以多拷贝形式存在。每个片段通过切口平移进行标记，并用于探测整个生殖支原体基因组DNA。大约一半的基因以单拷贝形式存在，而其余序列为多拷贝。单拷贝和多拷贝区域在整个结构基因中相互穿插。
