Tham T N, Ferris S, Bahraoui E, Canarelli S, Montagnier L, Blanchard A
Departement du SIDA et des Rétrovirus, Oncologie Virale, Institut Pasteur, Paris, France.
J Bacteriol. 1994 Feb;176(3):781-8. doi: 10.1128/jb.176.3.781-788.1994.
A DNA fragment has been isolated from the genome of Mycoplasma pirum by use of a genetic probe derived from the conserved region within the genes for the major adhesins of Mycoplasma genitalium and Mycoplasma pneumoniae. A gene encoding an adhesin-like polypeptide was localized, and sequence analysis indicated a G + C content of only 28%, with A- and T-rich codons being preferentially used. A total of 91% of positions 3 were either A or T. The deduced polypeptide is 1,144 amino acids long (126 kDa) and shows 26% identity with the adhesins of M. genitalium and M. pneumoniae. Other features in common with these two membrane proteins include a similar hydropathic profile and a proline-rich C terminus. Antibodies were prepared by using as an immunogen a peptide derived from the C terminus of the M. pirum adhesin-like polypeptide and were found to recognize on immunoblots a 126-kDa polypeptide from an M. pirum cellular extract. The characterization of the adhesin-like gene is a first step toward a better understanding of the mechanisms allowing this human mycoplasma to attach to host cells.
通过使用源自生殖支原体和肺炎支原体主要黏附素基因保守区域的基因探针，从梨支原体基因组中分离出了一个DNA片段。定位了一个编码黏附素样多肽的基因，序列分析表明其G + C含量仅为28%，优先使用富含A和T的密码子。第3位的所有位置中共有91%为A或T。推导的多肽长1144个氨基酸（126 kDa），与生殖支原体和肺炎支原体的黏附素具有26%的同一性。与这两种膜蛋白的其他共同特征包括相似的亲水性图谱和富含脯氨酸的C末端。通过使用源自梨支原体黏附素样多肽C末端的肽作为免疫原制备了抗体，发现这些抗体在免疫印迹中能识别梨支原体细胞提取物中的一种126 kDa多肽。对黏附素样基因的表征是朝着更好地理解这种人类支原体附着于宿主细胞的机制迈出的第一步。
