Noda D, Hamachi T, Inoue K, Maeda K
Section of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Higashi-ku, Fukuoka, Japan.
J Periodontal Res. 2007 Dec;42(6):566-71. doi: 10.1111/j.1600-0765.2007.00984.x.
Periodontal disease is a chronic disease characterized by the interaction between periodontopathic bacteria and the host immune response. The aim of this study was to investigate the correlation between periodontopathic bacteria and host immune cell infiltrates.
Twenty-two patients with chronic periodontitis were included in this study. Gingival tissues were taken at the periodontal surgery after completion of initial therapy. Three types of periodontopathic bacteria were detected by polymerase chain reaction, and the prevalence of mRNA expression of chemokine receptors was examined by reverse-transcription-polymerase chain reaction in the gingival tissues. The infiltration of T and B cells was determined by an immunohistochemical method.
In the patients, both Porphyromonas gingivalis and Tanerella forsythia were detected, and the mRNA expression of chemokine receptors CXCR1&2, CXCR4, CCR1, CCR2, CCR3 and CCR4 were more prevalent. The mean number of infiltrated B cells was significantly larger than that of T cells in the sites harboring both P. gingivalis and T. forsythia. Similarly, in the sites where P. gingivalis was detected but T. forsythia was not, the mean number of B cells was significantly larger than that of T cells. In the sites with mRNA expression of CCR2 and CCR3, the mean number of B cells was significantly larger.
These results suggest that a high proportion of T helper 2-associated chemokine receptor-positive T cells may be associated with the predominance of B cells and may play an important role in the formation of chronic periodontitis in sites where both P. gingivalis and T. forsythia are detected.
牙周病是一种以牙周病原菌与宿主免疫反应相互作用为特征的慢性疾病。本研究旨在探讨牙周病原菌与宿主免疫细胞浸润之间的相关性。
本研究纳入22例慢性牙周炎患者。在初始治疗完成后，于牙周手术时采集牙龈组织。采用聚合酶链反应检测三种牙周病原菌，并通过逆转录-聚合酶链反应检测牙龈组织中趋化因子受体mRNA表达的发生率。采用免疫组织化学方法测定T细胞和B细胞的浸润情况。
在患者中，牙龈卟啉单胞菌和福赛坦氏菌均被检测到，趋化因子受体CXCR1&2、CXCR4、CCR1、CCR2、CCR3和CCR4的mRNA表达更为普遍。在同时存在牙龈卟啉单胞菌和福赛坦氏菌的部位，浸润B细胞的平均数量显著多于T细胞。同样，在检测到牙龈卟啉单胞菌但未检测到福赛坦氏菌的部位，B细胞的平均数量也显著多于T细胞。在CCR2和CCR3 mRNA表达的部位，B细胞的平均数量显著增多。
这些结果表明，高比例的辅助性T细胞2相关趋化因子受体阳性T细胞可能与B细胞的优势有关，并且可能在同时检测到牙龈卟啉单胞菌和福赛坦氏菌的部位慢性牙周炎的形成中起重要作用。
