Ramachandran J, Lee V
Biochim Biophys Acta. 1976 Apr 23;428(2):339-46. doi: 10.1016/0304-4165(76)90041-6.
The stimulation of lipolysis in isolated rat and rabbit fat cells by adrenocorticotropin (ACTH) and alpha-melanotropin has been studied. The concentration of alpha-melanotropin required for half maximal stimulation is 0.23 times that of ACTH in rabbit adipocytes but as high as 1140 times that of ACTH in rat fat cells. Chemical modification of the tryptophan residue in ACTH and melanotropin resulted in a loss of lipolytic activity in rat adipocytes and an increase in lipolytic potency in rabbit fat cells. These differences between rat and rabbit fat cells were evident when stimulation of cyclic AMP synthesis was measured in isolated cells or ghosts. The results are discussed in terms of the difference in the hormone receptors of the fat cells of the two species.
已经研究了促肾上腺皮质激素（ACTH）和α-促黑素对分离的大鼠和兔脂肪细胞中脂解作用的刺激。在兔脂肪细胞中，达到最大刺激作用一半时所需的α-促黑素浓度是ACTH的0.23倍，但在大鼠脂肪细胞中则高达ACTH的1140倍。对ACTH和促黑素中色氨酸残基进行化学修饰后，大鼠脂肪细胞中的脂解活性丧失，而兔脂肪细胞中的脂解效力增加。当在分离的细胞或细胞膜空壳中测量环磷酸腺苷（cAMP）合成的刺激情况时，大鼠和兔脂肪细胞之间的这些差异很明显。根据这两个物种脂肪细胞中激素受体的差异对结果进行了讨论。
