Farjanel J, Pichat L, Audinot M, Perier C, Frey J
Biochimie. 1976;58(3):269-73. doi: 10.1016/s0300-9084(76)80433-6.
By use of procollagen proline hydroxylas which catalyses the substitution of the hydrogen in the trans position on carbon 4 with an hydroxyl on L-proline residues of procollagen, we have shown that the L-[3H-4] proline product of C.E.N. Saclay contained 29 per cent of the trans and 71 per cent of the cis form. It was also possible to verify the molecular stability of the isomers and that a small amount of tritium was randomly linked to the whole molecule.
通过使用催化原胶原蛋白L-脯氨酸残基上碳4位反式氢被羟基取代的原胶原蛋白脯氨酸羟化酶，我们已表明萨克莱中央核能研究中心的L-[3H-4]脯氨酸产物含有29%的反式形式和71%的顺式形式。还能够验证这些异构体的分子稳定性以及少量氚随机连接到整个分子上。
