在负透镜补偿和恢复过程中巩膜蛋白聚糖mRNA水平的选择性调节。

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery.

作者信息

Siegwart John Thomas, Strang Christianne E

机构信息

Department of Vision Sciences, School of Optometry, The University of Alabama at Birmingham, Birmingham, AL, USA.

出版信息

Mol Vis. 2007 Oct 4;13:1878-86.

PMID:17960126

Abstract

PURPOSE

To better characterize the role of proteoglycans in scleral tissue remodeling during the development of minus lens induced myopia and during recovery in tree shrews.

METHODS

Competitive reverse-transcription polymerase chain reaction (RT-PCR) was used to quantify the scleral mRNA levels for aggrecan, decorin, biglycan, and lumican in a group of tree shrews following four days of monocular -5 D lens treatment (n=5) and in a group after two days of recovery after 11 days of -5 D lens wear (n=5). Values were compared with age-matched normal animals (n=5). Aggrecan was localized within the sclera using immunohistochemistry.

RESULTS

Four days of -5 D lens wear produced axial (vitreous chamber) elongation and a myopic shift in the treated eyes. Two days of recovery produced significant refractive recovery. Aggrecan mRNA levels showed differential, bidirectional regulation. Levels in the treated eye sclera relative to the control eye were 30.8%+/-2.4% lower after four days of -5 D lens treatment and 51.4%+/-2.4% higher after two days of recovery. Decorin, biglycan, and lumican mRNA levels showed little differential regulation. However, biglycan and lumican along with aggrecan showed binocular regulation (treated and control eye mRNA levels significantly lower than normal eye mRNA levels after 4 days of -5D lens treatment). Immunohistochemical results showed that aggrecan is present in tree shrew sclera and that it is located primarily between the collagen lamella and near the fibroblasts.

CONCLUSIONS

These data suggest that the expression of aggrecan is strongly differentially modulated in the sclera during experimentally induced myopia and recovery. The modulation of aggrecan in concert with previously described changes in type I collagen and hyaluronan may play a key functional role in modulating the ability of the lamellae to slip across one another. This may be manifested in the scleral creep rate, which in turn modulates axial elongation rate and refractive state.

摘要

目的

更好地描述蛋白聚糖在树鼩近视性晶状体诱导性近视发展过程及恢复过程中巩膜组织重塑中的作用。

方法

采用竞争性逆转录聚合酶链反应（RT-PCR）定量一组树鼩在单眼-5D晶状体处理4天后（n=5）以及在-5D晶状体佩戴11天后恢复2天的一组树鼩中聚集蛋白聚糖、核心蛋白聚糖、双糖链蛋白聚糖和光蛋白聚糖的巩膜mRNA水平。将这些值与年龄匹配的正常动物（n=5）进行比较。使用免疫组织化学方法将聚集蛋白聚糖定位在巩膜内。

结果

-5D晶状体佩戴4天导致处理眼的眼轴（玻璃体腔）伸长和近视性偏移。恢复2天产生了显著的屈光恢复。聚集蛋白聚糖mRNA水平显示出差异双向调节。在-5D晶状体处理4天后，处理眼巩膜中的水平相对于对照眼降低了30.8%±2.4%，在恢复2天后升高了51.4%±2.4%。核心蛋白聚糖、双糖链蛋白聚糖和光蛋白聚糖mRNA水平显示出很少的差异调节。然而，双糖链蛋白聚糖、光蛋白聚糖以及聚集蛋白聚糖显示出双眼调节（在-5D晶状体处理4天后，处理眼和对照眼的mRNA水平显著低于正常眼的mRNA水平）。免疫组织化学结果显示，聚集蛋白聚糖存在于树鼩巩膜中，并且主要位于胶原板层之间和成纤维细胞附近。