Lin Xiao, Wang Bing-Jie, Wang Yen-Chiao, Chu Ren-Yuan, Dai Jin-Hui, Zhou Xing-Tao, Qu Xiao-Mei, Liu Hong, Zhou Hao
Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China.
Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China.
Int J Ophthalmol. 2018 Mar 18;11(3):354-362. doi: 10.18240/ijo.2018.03.02. eCollection 2018.
To address the microstructure and biomechanical changes of the sclera of rabbits after negative lens application by spectacle frame apparatus.
Five New Zealand rabbits of seven weeks post-natal were treated with -8 D lens monocularly over the course of two weeks. Refractive errors and axial length (AXL) were measured at the 1, 7 and 14 days of the induction period. Ultrastructure of sclera was determined with electron microscopy. Biomechanical properties were tested by an Instron 5565 universal testing machine.
Lens-induced (LI) eyes elongated more rapidly compared with fellow eyes with AXL values of 15.56±0.14 and 15.21±0.14 mm (<0.01). Fibril diameter was significantly smaller in the LI eyes compared with control ones in the inner, middle, and outer layers (inner layer, 63.533 76.467 nm; middle layer, 92.647 123.984 nm; outer layer, 86.999 134.257 nm, <0.01, respectively). In comparison with control eyes, macrophage-like cells that engulfed fibroblasts, dilated endoplasmic reticulum, and vacuoles in fibroblasts were observed in the inner and middle stroma in the LI eyes. Ultimate stress and Young's modulus were lower in the LI eyes compared with those in the control eyes.
Negative lens application alters eye growth, and results in axial elongation with changes in scleral ultrastructural and mechanical properties.
通过眼镜架装置施加负透镜，探讨兔巩膜的微观结构和生物力学变化。
对5只出生后7周的新西兰兔单眼施加-8D透镜，持续两周。在诱导期的第1、7和14天测量屈光不正和眼轴长度（AXL）。用电子显微镜观察巩膜的超微结构。用Instron 5565万能试验机测试生物力学性能。
与对侧眼相比，透镜诱导（LI）眼的眼轴伸长更快，AXL值分别为15.56±0.14和15.21±0.14mm（P<0.01）。LI眼内、中、外层的纤维直径均显著小于对照眼（内层，63.533±76.467nm；中层，92.647±123.984nm；外层，86.999±134.257nm，P均<0.01）。与对照眼相比，LI眼的内、中基质中可见吞噬成纤维细胞的巨噬细胞样细胞、扩张的内质网和成纤维细胞中的空泡。LI眼的极限应力和杨氏模量均低于对照眼。
施加负透镜可改变眼球生长，导致眼轴伸长，并伴有巩膜超微结构和力学性能的改变。
