Mattsson J G, Insulander M, Lebbad M, Björkman C, Svenungsson B
Department of Parasitology (SWEPAR), National Veterinary Institute and Swedish University of Agricultural Sciences, Uppsala, Sweden.
Epidemiol Infect. 2008 Aug;136(8):1147-52. doi: 10.1017/S0950268807009673. Epub 2007 Oct 26.
An outbreak of cryptosporidiosis associated with exposure to outdoor swimming-pool water affected an estimated 800-1000 individuals. PCR products were obtained from faecal specimens from 30 individuals who tested positive for Cryptosporidium oocysts. RFLP and sequencing analyses showed that all individuals were infected with Cryptosporidium parvum. Among the infected individuals, five had just swum in an adjacent indoor pool during the same period, and had no identified contact with individuals linked to the outdoor pool. With the use of subgenotyping based on analysis of three mini- and microsatellite loci, MS1, TP14, and GP15, we could identify two sources of exposure. One subtype was associated with the outdoor pool and another with the indoor pool. These data demonstrate that the use of mini- and microsatellite loci as markers for molecular fingerprinting of C. parvum isolates are valuable in the epidemiological investigation of outbreaks.
一起与接触室外游泳池水相关的隐孢子虫病暴发估计影响了800 - 1000人。从30名隐孢子虫卵囊检测呈阳性的个体的粪便标本中获得了PCR产物。限制性片段长度多态性（RFLP）和测序分析表明，所有个体均感染了微小隐孢子虫。在受感染个体中，有5人在同一时期刚在相邻的室内游泳池游泳，且未发现与室外游泳池相关人员有接触。通过基于对三个微卫星和小卫星位点MS1、TP14和GP15的分析进行亚基因分型，我们能够确定两个暴露源。一种亚型与室外游泳池相关，另一种与室内游泳池相关。这些数据表明，使用微卫星和小卫星位点作为微小隐孢子虫分离株分子指纹识别的标记物，在暴发的流行病学调查中具有重要价值。
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