Chan S Y
Department of Zoology, University of Hong Kong.
Prostaglandins. 1991 Oct;42(4):321-36. doi: 10.1016/0090-6980(91)90081-p.
The effects of exogenous prostaglandin (PG) E2 and F2 alpha on the morphology and lactate dehydrogenase (LDH) activities of pre-implantation mouse embryos in vitro were studied. A 24-hour exposure from 0.01 to 10 micrograms/ml of PGE2 at the 4-cell or morula stages had no effect on the morphology of embryos during the 144 hours in culture. Exposure to 10 micrograms/ml PGE2 at the blastocyst stage accelerated and enhanced spreading of the trophoblast in vitro. Embryos treated at 0.01 to 10 micrograms/ml PGE2 at various stages all showed a more rapid decline in LDH activity from morula to blastocysts. Treatment with 50 or 100 micrograms/ml PGE2 led to abnormal morphology of embryos in vitro. In contrast, continuous treatment with 0.01 to 100 micrograms/ml PGF2 alpha from 4-cell to early post-implantation (day 8) had no effect on the morphology of embryos, although breakdown of LDH was again accelerated. These results suggest that the peak of PGE2 secretion on day 4 of pregnancy in mice may enhance trophoblastic outgrowth, and the lower levels of PGE2 and PGF2 alpha secreted earlier in pregnancy modulate the development of pre-implantation mouse embryos.
研究了外源性前列腺素（PG）E2和F2α对体外培养的植入前小鼠胚胎形态及乳酸脱氢酶（LDH）活性的影响。在4细胞期或桑葚胚期，用0.01至10微克/毫升的PGE2处理24小时，对培养144小时内胚胎的形态没有影响。在囊胚期用10微克/毫升的PGE2处理，可加速并增强体外滋养层的铺展。在不同阶段用0.01至10微克/毫升的PGE2处理的胚胎，从桑葚胚到囊胚阶段，LDH活性均显示出更快的下降。用50或100微克/毫升的PGE2处理会导致体外胚胎形态异常。相比之下，从4细胞期到植入后早期（第8天）持续用0.01至100微克/毫升的PGF2α处理，对胚胎形态没有影响，尽管LDH的分解再次加速。这些结果表明，小鼠妊娠第4天PGE2分泌的峰值可能会增强滋养层的生长，而妊娠早期分泌的较低水平的PGE2和PGF2α则调节植入前小鼠胚胎的发育。
