Chida S, Uehara S, Hoshiai H, Yajima A
Prostaglandins. 1986 Feb;31(2):337-42. doi: 10.1016/0090-6980(86)90058-4.
The present study has been performed to investigate how PGs would participate the hatching process. Effects of indomethacin, an antagonist to PGs biosynthesis, on the hatching of mouse blastocysts were examined in vitro. Furthermore, it was studied that prostaglandin E2 (PGE2), prostaglandin F2 alpha (PGF2 alpha) or 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) were added to the culture media with indomethacin. The hatching was inhibited by indomethacin yet the inhibition was reversible. In the groups with indomethacin and PGE2, no improvement was seen in the inhibition of hatching and the inhibition was irreversible. In the groups with indomethacin and PGF2 alpha, inhibition of hatching was improved in comparison with the group with indomethacin. In the groups with indomethacin and 6-keto-PGF1 alpha, no improvement was seen. The above results indicated that PGF2 alpha possibly had an accelerating effect on hatching and a high concentration of PGE2 would exert cytotoxic effect on blastocysts.
本研究旨在探讨前列腺素（PGs）如何参与孵化过程。在体外检测了PGs生物合成拮抗剂吲哚美辛对小鼠囊胚孵化的影响。此外，还研究了将前列腺素E2（PGE2）、前列腺素F2α（PGF2α）或6-酮-前列腺素F1α（6-酮-PGF1α）添加到含有吲哚美辛的培养基中的情况。吲哚美辛抑制了孵化，但这种抑制是可逆的。在含有吲哚美辛和PGE2的组中，孵化抑制没有改善，且抑制是不可逆的。在含有吲哚美辛和PGF2α的组中，与仅含吲哚美辛的组相比，孵化抑制有所改善。在含有吲哚美辛和6-酮-PGF1α的组中，未见改善。上述结果表明，PGF2α可能对孵化有促进作用，而高浓度的PGE2会对囊胚产生细胞毒性作用。
