Whitney Elizabeth R, Kemper Thomas L, Rosene Douglas L, Bauman Margaret L, Blatt Gene J
Department of Anatomy and Neurobiology, L-1004, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA.
J Neurosci Methods. 2008 Feb 15;168(1):42-7. doi: 10.1016/j.jneumeth.2007.09.009. Epub 2007 Sep 16.
In a study of human Purkinje cell (PC) number, a striking mismatch between the number of PCs observed with the Nissl stain and the number of PCs immunopositive for calbindin-D28k (CB) was identified in 2 of the 10 brains examined. In the remaining eight brains this mismatch was not observed. Further, in these eight brains, analysis of CB immunostained sections counterstained with the Nissl stain revealed that more than 99% Nissl stained PCs were also immunopositive for CB. In contrast, in the two discordant brains, only 10-20% of CB immunopositive PCs were also identified with the Nissl stain. Although this finding was unexpected, a historical survey of the literature revealed that Spielmeyer [Spielmeyer W. Histopathologie des nervensystems. Julius Springer: Berlin; 1922. p. 56-79] described human cases with PCs that lacked the expected Nissl staining intensity, an important historical finding and critical issue when studying postmortem human brains. The reason for this failure in Nissl staining is not entirely clear, but it may result from premortem circumstances since it is not accounted for by postmortem delay or processing variables. Regardless of the exact cause, these observations suggest that Nissl staining may not be a reliable marker for PCs and that CB is an excellent alternative marker.
在一项关于人类浦肯野细胞（PC）数量的研究中，在所检查的10个大脑中的2个中，发现用尼氏染色观察到的PC数量与钙结合蛋白-D28k（CB）免疫阳性的PC数量之间存在显著不匹配。在其余8个大脑中未观察到这种不匹配。此外，在这8个大脑中，对用尼氏染色复染的CB免疫染色切片进行分析发现，超过99%的尼氏染色PC对CB也呈免疫阳性。相比之下，在两个不一致的大脑中，只有10%-20%的CB免疫阳性PC也能用尼氏染色鉴定出来。尽管这一发现出乎意料，但对文献的历史调查显示，施皮尔曼[施皮尔曼W. 神经系统组织病理学。朱利叶斯·施普林格出版社：柏林；1922年。第56 - 79页]描述了人类PC缺乏预期尼氏染色强度的病例，这是一个重要的历史发现，也是研究死后人类大脑时的关键问题。尼氏染色失败的原因尚不完全清楚，但可能是由于生前情况所致，因为死后延迟或处理变量无法解释这一现象。无论确切原因如何，这些观察结果表明，尼氏染色可能不是PC的可靠标记，而CB是一种优秀的替代标记。
