Johnson E H, Lustigman S, Brotman B, Browne J, Prince A M
Laboratory of Virology and Parasitology, Lindsley F. Kimball Research Institute, New York Blood Center, New York.
Trop Med Parasitol. 1991 Dec;42(4):351-5.
Peripheral blood leukocytes from chimpanzees experimentally infected with third stage larvae, or with nodular microfilaria (mf) of Onchocerca volvulus, were evaluated for their ability to kill nodular mf in vitro. Inoculation of chimpanzees with third stage larvae, and prospective follow up, permitted each animal to be classified as patent, infected but not patent, or post-patent. Neither lymphocytes nor monocytes significantly adhered to, immobilized, or killed mf. However, neutrophils or eosinophils killed mf effectively in the presence of immune serum. Neutrophil and eosinophil enriched cell preparations from control animals were able to kill mf with serum from infected animals, but to a significantly lesser degree than when both cells and serum were taken from infected animals. Chimpanzees inoculated with mf showed a progressive increase in cellular killing of mf after a second inoculation which surpassed that seen with animals inoculated with third stage larvae. Antibody-mediated killing of mf by neutrophils and eosinophils may be important in the termination of patency in this infection.
对实验感染了盘尾丝虫第三期幼虫或结节性微丝蚴（mf）的黑猩猩的外周血白细胞进行体外杀灭结节性微丝蚴能力的评估。用第三期幼虫接种黑猩猩并进行前瞻性随访，可将每只动物分为有虫血症、感染但无虫血症或虫血症后阶段。淋巴细胞和单核细胞均未显著黏附、固定或杀死微丝蚴。然而，在免疫血清存在的情况下，中性粒细胞或嗜酸性粒细胞能有效杀死微丝蚴。来自对照动物的富含中性粒细胞和嗜酸性粒细胞的细胞制剂能够用感染动物的血清杀死微丝蚴，但程度明显低于细胞和血清均取自感染动物时。接种微丝蚴的黑猩猩在第二次接种后对微丝蚴的细胞杀伤作用逐渐增强，超过了接种第三期幼虫的动物。中性粒细胞和嗜酸性粒细胞通过抗体介导对微丝蚴的杀伤作用在这种感染的虫血症终止过程中可能很重要。
