Carpagnano Giovanna E, Foschino-Barbaro Maria Pia, Spanevello Antonio, Resta Onofrio, Carpagnano Francesco, Mulé Giuseppina, Pinto Rosamaria, Tommasi Stefania, Paradiso Angelo
Institute of Respiratory Disease, University of Foggia, Foggia, Italy.
Am J Respir Crit Care Med. 2008 Feb 1;177(3):337-41. doi: 10.1164/rccm.200707-1136OC. Epub 2007 Oct 25.
Our group has recently demonstrated the possibility of studying microsatellite alterations (MAs) of 3p in the DNA of exhaled breath condensate (EBC) of patients with non-small cell lung cancer (NSCLC).
To verify whether MAs analyzed in DNA from EBC reflect a profile of alterations present in tumor tissue of NSCLC.
Fifty-nine subjects undergoing histologic diagnosis for clinical suspicion of lung cancer entered the study: 41 were found to have NSCLC and 18 to have nonneoplastic diseases. All subjects underwent allelotyping on DNA from whole blood, EBC, and lung tissue removed for histologic diagnosis by analyzing a panel of five microsatellites located in chromosomal region 3p. Results obtained from DNA of the three biological sites and nonneoplastic tissues from controls were compared.
MAs in DNA from tumor tissues and EBC of each patient with cancer presented an overlapping profile of loss of heterozygosity and microsatellite instability. An MA profile of DNA of lung tissue reflecting the DNA of EBC profile from controls was also confirmed. Smoking status was associated with the presence of MAs in patients with NSCLC and in control subjects.
We demonstrated that MAs in DNA from EBC of patients with NSCLC are significantly more frequent than in control subjects. More interesting, the MA profile of DNA from EBC corresponds to that from lung cancer tissue of each patient with NSCLC.
我们的研究小组最近证明了在非小细胞肺癌（NSCLC）患者呼出气冷凝液（EBC）的DNA中研究3p微卫星改变（MA）的可能性。
验证从EBC的DNA中分析出的MA是否反映了NSCLC肿瘤组织中存在的改变特征。
59名因临床怀疑肺癌而接受组织学诊断的受试者进入本研究：41名被诊断为NSCLC，18名患有非肿瘤性疾病。所有受试者均通过分析位于染色体区域3p的一组五个微卫星，对全血、EBC和用于组织学诊断而切除的肺组织的DNA进行基因分型。比较了从这三个生物学部位的DNA以及对照组非肿瘤组织获得的结果。
每位癌症患者肿瘤组织和EBC的DNA中的MA呈现出杂合性缺失和微卫星不稳定性的重叠特征。还证实了反映对照组EBC DNA特征的肺组织DNA的MA特征。吸烟状态与NSCLC患者和对照受试者中MA的存在有关。
我们证明，NSCLC患者EBC的DNA中的MA比对照受试者明显更频繁。更有趣的是，EBC的DNA的MA特征与每位NSCLC患者肺癌组织的MA特征相对应。
