PDZ-LIM蛋白CLP36是BeWo细胞中肌动蛋白应力纤维形成和粘着斑组装所必需的。
The PDZ-LIM protein CLP36 is required for actin stress fiber formation and focal adhesion assembly in BeWo cells.
作者信息
Tamura Naoaki, Ohno Koji, Katayama Taiichi, Kanayama Naohiro, Sato Kohji
机构信息
Department of Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashiku, Hamamatsu, 431-3192 Shizuoka, Japan.
出版信息
Biochem Biophys Res Commun. 2007 Dec 21;364(3):589-94. doi: 10.1016/j.bbrc.2007.10.064. Epub 2007 Oct 22.
CLP36 belongs to the ALP subfamily of PDZ-LIM proteins and has a PDZ domain at its N-terminal and a LIM domain at its C-terminal. It has been shown that CLP36 is localized to stress fibers through interaction with alpha-actinin, but its function is still unclear. To investigate the role of CLP36 in stress fibers, we suppressed CLP36 expression in BeWo cells by RNAi and examined the phenotypic changes. CLP36-knockdown resulted in cell spreading and the loss of stress fibers and focal adhesions. These changes were reversed by addition of exogenous CLP36, but not by addition of mutant forms of CLP36 that lacked the PDZ or LIM domain. These findings indicate that CLP36 plays a critical role in stress fiber formation and the assembly of focal adhesions in BeWo cells. In addition, the PDZ and LIM domains are both essential for CLP36 to function.
CLP36属于PDZ-LIM蛋白的碱性磷酸酶亚家族，其N端有一个PDZ结构域，C端有一个LIM结构域。研究表明，CLP36通过与α-辅肌动蛋白相互作用定位于应力纤维，但其功能仍不清楚。为了研究CLP36在应力纤维中的作用，我们通过RNA干扰抑制了BeWo细胞中CLP36的表达，并检测了表型变化。CLP36基因敲低导致细胞铺展以及应力纤维和粘着斑的丧失。添加外源性CLP36可逆转这些变化，但添加缺乏PDZ或LIM结构域的CLP36突变体则不能。这些发现表明，CLP36在BeWo细胞的应力纤维形成和粘着斑组装中起关键作用。此外，PDZ和LIM结构域对CLP36发挥功能都至关重要。
Zotero 插件