锡兰肉桂超临界二氧化碳提取物：化学表征及抗酪氨酸酶活性

Supercritical CO2 extract of Cinnamomum zeylanicum: chemical characterization and antityrosinase activity.

作者信息

Marongiu Bruno, Piras Alessandra, Porcedda Silvia, Tuveri Enrica, Sanjust Enrico, Meli Massimo, Sollai Francesca, Zucca Paolo, Rescigno Antonio

机构信息

Dipartimento di Scenze Chimiche, Università di Cagliari Cittadella Universitaria, Monserrato, Italy.

出版信息

J Agric Food Chem. 2007 Nov 28;55(24):10022-7. doi: 10.1021/jf071938f. Epub 2007 Oct 30.

DOI:10.1021/jf071938f

PMID:17966976

Abstract

The volatile oil of the bark of Cinnamomum zeylanicum was extracted by means of supercritical CO2 fluid extraction in different conditions of pressure and temperature. Its chemical composition was characterized by GC-MS analysis. Nineteen compounds, which in the supercritical extract represented >95% of the oil, were identified. (E)-Cinnamaldehyde (77.1%), (E)-beta-caryophyllene (6.0%), alpha-terpineol (4.4%), and eugenol (3.0%) were found to be the major constituents. The SFE oil of cinnamon was screened for its biological activity about the formation of melanin in vitro. The extract showed antityrosinase activity and was able to reduce the formation of insoluble flakes of melanin from tyrosine. The oil also delayed the browning effect in apple homogenate. (E)-Cinnamaldehyde and eugenol were found to be mainly responsible of this inhibition effect.

摘要

采用超临界CO2流体萃取法，在不同压力和温度条件下，从锡兰肉桂树皮中提取挥发油。通过气相色谱-质谱联用（GC-MS）分析对其化学成分进行表征。鉴定出19种化合物，这些化合物在超临界提取物中占挥发油的比例超过95%。发现（E）-肉桂醛（77.1%）、（E）-β-石竹烯（6.0%）、α-松油醇（4.4%）和丁香酚（3.0%）是主要成分。对肉桂的超临界流体萃取（SFE）油进行了体外黑色素形成相关生物活性的筛选。该提取物显示出抗酪氨酸酶活性，并且能够减少酪氨酸形成不溶性黑色素片。该油还延缓了苹果匀浆的褐变效应。发现（E）-肉桂醛和丁香酚是这种抑制作用的主要原因。