他汀类药物对腹主动脉瘤壁蛋白水解活性的影响。

Effect of statins on proteolytic activity in the wall of abdominal aortic aneurysms.

作者信息

Abisi S, Burnand K G, Humphries J, Waltham M, Taylor P, Smith A

机构信息

Academic Department of Surgery, Cardiovascular Division, King's College School of Medicine, London, UK.

出版信息

Br J Surg. 2008 Mar;95(3):333-7. doi: 10.1002/bjs.5989.

DOI:10.1002/bjs.5989

PMID:17968978

Abstract

BACKGROUND

The aim of this study was to examine the effect of statin treatment on the activity of proteases in the wall of abdominal aortic aneurysms (AAAs).

METHODS

The activities of matrix metalloproteinases (MMPs) 9 and 3, cathepsins B, H, K, L and S, and the cystatin C level were measured in extracts of AAA wall taken from 82 patients undergoing AAA repair; 21 patients were receiving statin treatment before surgery. All values were standardized against soluble protein (SP) concentration in the extract, and reported as median (interquartile range) or mean(s.e.m.).

RESULTS

The two groups had similar demographics. Reduced activity of MMP-9 (43 (34-56) versus 80 (62-110) pg per mg SP; P < 0.001), cathepsin H (183 (117-366) versus 321 (172-644) nmol 4-methylcoumarin-7-amide released per mg SP; P = 0.016) and cathepsin L (102 (51-372) versus 287 (112-816) micromol 7-amino-4-trifluoromethylcoumarin released per mg SP; P = 0.020) was found in the statin-treated aortas compared with AAAs from patients not taking a statin. The statin-treated group had lower MMP-3 activity, but this did not reach statistical significance (P = 0.053). Cystatin C levels were higher in statin-treated aortas than in controls (41.3(3.1) versus 28.9(2.1) ng per mg SP; P = 0.003).

CONCLUSION

Statins decreased the activity of proteases that have been implicated in aneurysm disease.

摘要

背景

本研究旨在探讨他汀类药物治疗对腹主动脉瘤（AAA）壁蛋白酶活性的影响。

方法

测量了82例行AAA修复术患者的AAA壁提取物中基质金属蛋白酶（MMP）9和3、组织蛋白酶B、H、K、L和S的活性以及胱抑素C水平；21例患者在手术前接受他汀类药物治疗。所有数值均根据提取物中的可溶性蛋白（SP）浓度进行标准化，并报告为中位数（四分位间距）或均值（标准误）。

结果

两组患者的人口统计学特征相似。与未服用他汀类药物患者的AAA相比，他汀类药物治疗的主动脉中MMP-9活性降低（每毫克SP为43（34-56）皮克对80（62-110）皮克；P<0.001）、组织蛋白酶H活性降低（每毫克SP释放的4-甲基香豆素-7-酰胺为183（117-366）纳摩尔对321（172-644）纳摩尔；P=0.016）以及组织蛋白酶L活性降低（每毫克SP释放的7-氨基-4-三氟甲基香豆素为102（51-372）微摩尔对287（112-816）微摩尔；P=0.020）。他汀类药物治疗组的MMP-3活性较低，但未达到统计学显著性（P=0.053）。他汀类药物治疗的主动脉中胱抑素C水平高于对照组（每毫克SP为41.3（3.1）纳克对28.9（2.1）纳克；P=0.003）。