Petrushanko I Iu, Zelenikhin P V, Mit'kevich V A, Kleĭmenova A A, Prasolov V S, Makarov A A, Il'inskaia O N
Biofizika. 2007 Sep-Oct;52(5):876-81.
The effect of cationic microbial ribonuclease from Bacillus intermedius (binase) on normal precursors of myeloid cells of FDC-P1 mice and kit-transformed precursors expressing the receptor of the growth factor of stem cells has been studied by flow-through cytometry. Selective apoptogenic properties of binase toward kit-transformed cells were revealed. Viable kit-transformed cells responded to binase by an increase in the concentration of cytosolic calcium. The content of calcium in the cytosol of both cell types in which apoptosis was induced by binase decreased in a dose-dependent manner. The death of cells was not accompanied by a substantial decrease in the content of intracellular RNA. A possible mechanism of binase-induced effects, which involves changes in the expression of genes due to the interference of exogenous RNAse into the RNA interference, was considered.
通过流式细胞术研究了来自中间芽孢杆菌的阳离子微生物核糖核酸酶（binase）对FDC-P1小鼠髓样细胞正常前体以及表达干细胞生长因子受体的kit转化前体的影响。揭示了binase对kit转化细胞的选择性凋亡特性。有活力的kit转化细胞对binase的反应是胞质钙浓度增加。binase诱导凋亡的两种细胞类型的胞质钙含量均呈剂量依赖性下降。细胞死亡并未伴随细胞内RNA含量的大幅降低。考虑了binase诱导效应的一种可能机制，即由于外源核糖核酸酶干扰RNA干扰而导致基因表达的变化。
