Garipov Azat R, Nesmelov Alexander A, Cabrera-Fuentes Hector A, Ilinskaya Olga N
Department of Microbiology, Kazan Federal (Volga-Region) University, Kremlevskaya str. 18, Kazan 420008, Russia.
Department of Microbiology, Kazan Federal (Volga-Region) University, Kremlevskaya str. 18, Kazan 420008, Russia; Institute of Biochemistry, Medical School, Justus-Liebig-University, Friedrichstrasse, 24, 35390 Giessen, Germany.
Toxicon. 2014 Dec 15;92:54-9. doi: 10.1016/j.toxicon.2014.09.014. Epub 2014 Oct 6.
The cytotoxic effects of Bacillus intermedius RNase (binase) towards ovarian cancer cells (SKOV3 and OVCAR5) were studied in comparison to normal ovarian epithelial cells (HOSE1 and HOSE2). Binase decreased viability and induced the selective apoptosis of ovarian cancer cells. The apoptosis rate was 50% in SKOV3 and 48% in OVCAR5 cells after 24 h of binase treatment (50 μg/ml). Binase-induced apoptosis in these cell lines was accompanied by caspase-3 activation and poly(ADP-ribose) polymerase fragmentation. Normal ovarian epithelial cells were not affected by binase, except for a slight decrease of HOSE2 cell viability and the appearance of traces of activated caspase-3, but not the poly(ADP-ribose) polymerase 85-kDA fragment. Binase did not induce alteration of EZH2 (enhancer of zeste-homolog-2) protein expression neither, in tumor nor in normal cells. In conclusion, selective binase-induced cell death and apoptosis via poly(ADP-ribose) polymerase fragmentation may serve as a new treatment option against ovarian cancer progression.
研究了中间芽孢杆菌核糖核酸酶（binase）对卵巢癌细胞（SKOV3和OVCAR5）的细胞毒性作用，并与正常卵巢上皮细胞（HOSE1和HOSE2）进行了比较。Binase降低了卵巢癌细胞的活力并诱导其选择性凋亡。在binase（50μg/ml）处理24小时后，SKOV3细胞的凋亡率为50%，OVCAR5细胞的凋亡率为48%。Binase诱导这些细胞系凋亡的同时伴有caspase-3激活和聚（ADP-核糖）聚合酶断裂。正常卵巢上皮细胞不受binase影响，除了HOSE2细胞活力略有下降以及出现微量活化的caspase-3，但没有85-kDa聚（ADP-核糖）聚合酶片段。Binase在肿瘤细胞和正常细胞中均未诱导EZH2（zeste同源物2增强子）蛋白表达改变。总之，通过聚（ADP-核糖）聚合酶断裂，binase诱导的选择性细胞死亡和凋亡可能成为对抗卵巢癌进展的一种新的治疗选择。
