Eide A, Fossberg T M
Cell Tissue Res. 1976 Jun 11;169(1):1-6. doi: 10.1007/BF00219302.
Uterine tissue from neonatal mice was incubated in vitro at 0 degrees C for 1 h in a medium containing 1 X 10(-8) M 3H-estradiol with or without 1 X 10(-4) M dibutyryl cyclic AMP. In some incubations the temperature was raised to 37 degrees C for 15 min after incubation in the cold, in others the temperature was kept at 0 degrees C during this 15 min period. The tissue was frozen in liquid propane cooled in liquid nitrogen, sectioned at 2 or 4 microns, and autoradiograms prepared according to the dry-mount procedure. cAMP increases the cellular uptake of 3H-estradiol in uterine tissue. After rising the temperature to 37 degrees C, grains appeared over the nuclei. cAMP at low temperature increased the cellular uptake of 3H-estradiol, but the grains were not associated with the nuclei. In the autoradiograms the grain number above the epithelium was markedly less than above the stroma.
将新生小鼠的子宫组织在含有1×10⁻⁸ M³H-雌二醇的培养基中于0℃体外孵育1小时，培养基中添加或不添加1×10⁻⁴ M二丁酰环磷酸腺苷。在一些孵育过程中，在冷孵育后将温度升至37℃持续15分钟，在另一些孵育过程中，在这15分钟期间温度保持在0℃。将组织在液氮冷却的液体丙烷中冷冻，切成2或4微米的切片，并根据干裱法制备放射自显影片。环磷酸腺苷增加子宫组织中³H-雌二醇的细胞摄取。将温度升至37℃后，细胞核上出现银粒。低温下的环磷酸腺苷增加了³H-雌二醇的细胞摄取，但银粒与细胞核无关。在放射自显影片中，上皮上方的银粒数量明显少于基质上方的银粒数量。
