Keefer D A
Horm Metab Res. 1982 Apr;14(4):209-12. doi: 10.1055/s-2007-1018971.
This investigation was carried out to examine the temporal aspects of nuclear retention of 3H-estrogen by individual target cell types of the rat uterus and pituitary. A quantitative dry autoradiographic-immunocytochemical method was used to measure relative 3H-estrogen uptake by nuclei of seven different target cell types 15 m, 1 h, 3 h and 7 h after injection of 3H-estradiol-17 beta. All five target cell types in anterior pituitary showed maximal nuclear retention at 15 m. Possible cell-type differences were noted in the rate of release of the isotope from nuclei. In the uterus maximal uptake, occurred between 1 h and 3 h after injection with stromal cells consistently concentrating more 3H-estrogen than luminal epithelial cells. These findings indicate possible differences between the mechanisms controlling nuclear retention of estrogen by the uterus and the pituitary.
本研究旨在探讨大鼠子宫和垂体的单个靶细胞类型对³H-雌激素核保留的时间方面。采用定量干放射自显影-免疫细胞化学方法，在注射³H-雌二醇-17β后15分钟、1小时、3小时和7小时，测量七种不同靶细胞类型细胞核对³H-雌激素的相对摄取量。垂体前叶的所有五种靶细胞类型在15分钟时显示出最大核保留。注意到同位素从细胞核释放速率可能存在细胞类型差异。在子宫中，最大摄取发生在注射后1至3小时之间，基质细胞始终比腔上皮细胞积累更多的³H-雌激素。这些发现表明子宫和垂体控制雌激素核保留的机制可能存在差异。
